Site-specific gene transfer with high efficiency onto a carbon nanotube-loaded electrode
Site-specific gene transfer with high efficiency onto a carbon nanotube-loaded electrode
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DOI：
10.1098/rsif.2007.1295
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发表时间：
2008-08-06
影响因子：
3.9
通讯作者：
Iwata, H.
中科院分区：
文献类型：
--
作者：
Inoue, Y.;Fujimoto, H.;Iwata, H.
转染芯片是一种专门用于高通量分析基因组功能的芯片，它通过在芯片上过表达或抑制基因而被开发，有望成为后基因组研究的一种重要方法。高效率的基因表达或抑制对于高通量分析是必不可少的，因为随着转染阵列中斑点密度的增加，单个斑点上的贴壁细胞数量减少。我们已经研究了一种电刺激孔形成的细胞膜上的基因传递。细胞膜上应形成细孔，以提高基因转染效率而不损伤细胞。在此，我们研究了在表面上携带化学官能化的碳纳米管（CNT）的电极。通过静电作用将碳纳米管负载在具有自组装单层膜的金电极上。吸附的质粒DNA转染到贴壁细胞上的CNT负载的电极比没有CNT的电极上的效率更高。这一结果可能是由于从碳纳米管的尖端形成的强而精细的场发射。
A transfection array, which is specifically developed for use in high-throughput analyses of genome functions by the over-expression or suppression of genes on a chip, is expected to become an important method for post-genome research. High efficiency of gene expression or suppression is indispensable for high-throughput analyses because the adherent cell number on a single spot decreases as the density of spots increases in the transfection array. We have studied an electro-stimulated pore formation on the cell membrane for gene delivery. Fine pores should be formed on the cell membrane to increase the efficiency of gene transfection without cell damage. Herein, we examined the electrode carrying chemically functionalized carbon nanotubes (CNTs) on the surface. The CNTs were loaded on a gold electrode with a self-assembled monolayer membrane by electrostatic interaction. Adsorbed plasmid DNA was transfected with higher efficiency into adherent cells on the CNT-loaded electrode than on an electrode without CNTs. This result may be due to the strong but fine field emission formed from the tips of the CNTs.