C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea
C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea
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DOI：
10.1126/science.281.5373.99
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发表时间：
1998-07-03
期刊：
影响因子：
56.9
通讯作者：
Lidstrom, ME
中科院分区：
文献类型：
--
作者：
Chistoserdova, L;Vorholt, JA;Lidstrom, ME
产甲烷菌和硫酸盐还原菌被认为具有涉及C-1转移辅酶和这组严格厌氧微生物特有的酶的能量代谢。一种好氧甲基营养菌，扭脱甲基杆菌AM 1，被发现含有一组基因，预测编码这些酶中的一些，并显示含有两种酶的活性和一种产甲烷辅酶。插入突变体都不能在C-1化合物上生长，表明古细菌酶在有氧C-1代谢中起作用。因此，甲基营养和甲烷生成涉及跨越细菌/古细菌边界的共同基因。
Methanogenic and sulfate-reducing Archaea are considered to have an energy metabolism involving C-1 transfer coenzymes and enzymes unique for this group of strictly anaerobic microorganisms. An aerobic methylotrophic bacterium, Methylobacterium extorquens AM1, was found to contain a cluster of genes that are predicted to encode some of these enzymes and was shown to contain two of the enzyme activities and one of the methanogenic coenzymes. Insertion mutants were all unable to grow on C-1 compounds, suggesting that the archaeal enzymes function in aerobic C-1 metabolism. Thus, methylotrophy and methanogenesis involve common genes that cross the bacterial/archaeal boundaries.