LOCALIZATION OF A PROTON-PUMPING ATPASE IN RAT-KIDNEY
LOCALIZATION OF A PROTON-PUMPING ATPASE IN RAT-KIDNEY
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DOI：
10.1172/jci113833
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发表时间：
1988-12-01
影响因子：
15.9
通讯作者：
GLUCK, S
中科院分区：
文献类型：
--
作者：
BROWN, D;HIRSCH, S;GLUCK, S
用免疫细胞化学方法检测了大鼠肾脏空泡H ~+-ATP酶的分布，用亲和纯化的牛肾质子泵31-，56-和70-kD亚基抗体。近曲小管顶端质膜内陷标记，并在薄降支的初始部分，顶端和基底侧质膜中度染色。粗的升支和远曲小管顶部染色，尽管远曲小管的强度更大。集合管主细胞几乎未标记，但闰细胞有强烈的染色与顶端，基底侧或弥漫性模式在皮质，和专门的顶端染色在髓质。这些结果（a）表明近端小管顶侧质膜中存在H+ ATP酶，可能有助于该节段的H+转运;（B）提供直接证据表明，闰细胞含有集合管中可检测到的大部分H+ ATP酶，支持其在H+转运中的拟议作用;（c）证明皮质闰细胞的亚群具有相反极性的H+ ATP酶，与质子和碳酸氢盐分泌细胞的存在一致;和（d）表明H+ ATP酶在集合管和近端小管以外的节段中的酸/碱调节或H+转运中的作用。
The distribution of vacuolar H+-ATPase in rat kidney was examined by immunocytochemistry using affinity-purified antibodies against the 31-, 56-, and 70-kD subunits of the bovine kidney proton pump. Proximal convoluted tubules were labeled over apical plasma membrane invaginations, and in the initial part of the thin descending limb, apical and basolateral plasma membranes were moderately stained. Thick ascending limbs and distal convoluted tubules were apically stained although the intensity was greater in the distal convoluted tubule. Collecting duct principal cells were virtually unlabeled, but intercalated cells had intense staining with an apical, basolateral or diffuse pattern in the cortex, and exclusively apical staining in the medulla. These results (a) show the presence of an H+ATPase in the apical plasma membrane of the proximal tubule that may contribute to H+ transport in this segment; (b) provide direct evidence that the intercalated cell contains most of the H+ATPase detectable in the collecting duct, supporting its proposed role in H+ transport; (c) demonstrate that subpopulations of cortical intercalated cells have opposite polarities of an H+ATPase, consistent with the presence of both proton- and bicarbonate-secreting cells; and (d) suggest a role for the H+ATPase in acid/base regulation or H+ transport in segments other than the collecting duct and the proximal tubule.