Visualisation of Microglia with the use of Immunohistochemical Double Staining Method for CD-68 and Iba-1 of Cerebral Tissue Samples in Cases of Brain Contusions.
Visualisation of Microglia with the use of Immunohistochemical Double Staining Method for CD-68 and Iba-1 of Cerebral Tissue Samples in Cases of Brain Contusions.
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DOI：
10.1515/prilozi-2015-0062
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Rosoklija, Gorazd
中科院分区：
文献类型：
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作者：
Stankov, Aleksandar;Belakaposka-Srpanova, Viktorija;Rosoklija, Gorazd
近年来，已经证实神经细胞损伤中免疫应答的主要成分来自小胶质细胞和巨噬细胞。小胶质细胞研究领域的主要挑战是通过细胞形态的可视化来检测细胞活化的不同阶段。现有的可视化技术是基于表面分子在静息和活化的小胶质细胞中的表达。为了观察不同存活时间脑挫伤中小胶质细胞及其功能状态，我们采用cd-68和iba 1双标记法。小胶质细胞用Iba-1染色为棕色，而浸渍有黑色颗粒状颜色的小胶质细胞代表用CD 68染色的活化的小胶质细胞。我们得到了显著的阳性结果，我们能够观察到与存活时间相关的小胶质细胞形态学变化。使用Iba-1和cd 68的双标记，我们能够根据形态学和免疫反应性确定它们的生理状态。
In the recent years it has been confirmed that the main component of the immune response in an injury of the nerve cell comes from microglia and macrophages. The main challenge in the field of microglia research is to detect the different stages of cellular activation by visualization of the cell morphology. The existing visualization techniques are based on surface molecules expression in resting and activated microglia cells. For visualization of the microglial cells and their functional state we used double labeling method for cd-68 and iba1 in brain contusions with different survival time. Microglia are stained brown with Iba-1, whereas microglia impregnated with black, grainy color, represents activated microglia stained with CD 68. We had significantly positive results, and we were able to observe changes in the morphology of the microglia that correlated with the survival time. Using double labeling with Iba-1 and cd68 we were able to determine their physiological state based on the morphology and immunoreactivity.