Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects.
Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects.
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DOI：
10.1364/ol.36.000202
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发表时间：
2011-01-15
期刊：
影响因子：
3.6
通讯作者：
Moerner WE
中科院分区：
文献类型：
--
作者：
Lew MD;Lee SF;Badieirostami M;Moerner WE
我们描述了螺旋点扩散函数 (PSF)，它可以在 3.2 µm 景深的三个维度上以纳米精度定位物体。螺旋形 PSF 根据发射器的轴向 (z) 位置进行旋转。 Fisher信息计算表明，螺旋PSF可以在有限的光子数量下实现纳米级的定位精度。我们通过在三角形聚二甲基硅氧烷 (PDMS) 光栅表面上成像珠子来演示使用螺旋 PSF 的三维超分辨率显微镜。螺旋形 PSF 检测到 99,000 个光子，其 x 轴定位精度为 2.7 nm，y 轴定位精度为 2.1 nm，z 轴定位精度为 5.7 nm。
We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 µm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z.