ANTI-GLYCOPROTEIN-D ANTIBODIES THAT PERMIT ADSORPTION BUT BLOCK INFECTION BY HERPES-SIMPLEX VIRUS-1 PREVENT VIRION CELL-FUSION AT THE CELL-SURFACE
ANTI-GLYCOPROTEIN-D ANTIBODIES THAT PERMIT ADSORPTION BUT BLOCK INFECTION BY HERPES-SIMPLEX VIRUS-1 PREVENT VIRION CELL-FUSION AT THE CELL-SURFACE
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DOI：
10.1073/pnas.84.15.5454
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
SPEAR, PG
中科院分区：
文献类型：
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作者：
FULLER, AO;SPEAR, PG
某些对单纯疱疹病毒糖蛋白 D 具有特异性的单克隆抗体具有有效的中和活性，但无法阻止病毒与细胞的附着。在这里，我们研究了中和和感染性病毒附着在灵长类细胞后的命运。感染性病毒颗粒与细胞表面融合，使得在质膜附近或紧邻质膜下方的细胞质中可以检测到裸露的核衣壳。中和的病毒粒子不与细胞融合。它们仍然附着在细胞表面，并且通过聚乙二醇处理可以使其具有感染性。我们得出结论，一些抗糖蛋白 D 中和抗体可以通过阻止病毒体包膜与质膜的融合来抑制单纯疱疹病毒的渗透。这些结果确定了启动成功的单纯疱疹病毒感染的进入途径以及该途径中对中和抗体高度敏感的步骤。表明了糖蛋白 D 在病毒粒子-细胞融合中的作用。
Certain monoclonal antibodies specific for glycoprotein D of herpes simplex virus have potent neutralizing activity but fail to block attachment of virus to cells. Here we have investigated the fate of neutralized and infectious virus after attachment to primate cells. Infectious virions fused with the cell surface such that naked nucleocapsids were detectable in the cytoplasm near or just under the plasma membrane. Neutralized virions did not fuse with the cell. They remained attached to the cell surface and could be rendered infectious by treatment with polyethylene glycol. We conclude that some anti-glycoprotein D neutralizing antibodies can inhibit the penetration of herpes simplex virus by blocking fusion of the virion envelope with the plasma membrane. These results identify a pathway of entry that initiates successful herpes simplex virus infection and a step in this pathway that is highly sensitive to neutralizing antibodies. A role for glycoprotein D in virion-cell fusion is indicated.