Glutamate stimulation increases hormone release in rat melanotrophs.
Glutamate stimulation increases hormone release in rat melanotrophs.
复制标题
谷氨酸刺激会增加大鼠黑色素细胞的激素释放。
DOI：
10.1016/j.neulet.2006.06.021
复制
发表时间：
2006
影响因子：
2.5
通讯作者：
Zorec,Robert
中科院分区：
文献类型：
--
作者：
Kreft,Marko;Blaganje,Mija;Grilc,Sonja;Rupnik,Marjan;Zorec,Robert
In melanotrophs, neuroendocrine cells from the intermediate lobe of the rat pituitary gland, glutamate causes a rise in intracellular [Ca2+] suggesting the presence of ionotropic NMDA and non-NMDA AMPA/K receptors. However, the Ca2+-dependent release of the major peptide hormone, α-melanocyte stimulating hormone (α-MSH), in response to glutamate stimulation has not been studied yet in this cell model. Significant spontaneous secretion of the peptide, which results in hormone deposits on the perimeter of the cells, has been confirmed by using confocal microscopy. Co-staining with a membrane area marker FM 1-43, which co-localized with the immunocytochemically marked hormone deposits, showed that fusion-competent sites on the plasma membrane coincided with secretion-competent sites. Stimulation of the cells with glutamate and high K+saline induced a significant increase in the plasma membrane area covered with α-MSH deposits compared to control cells incubated with glutamate and CNQX, a glutamate channel blocker. The optical approach to monitor the secretory activity of a single neuroendocrine cell revealed that glutamate stimulates the release of α-MSH at distinct exocytotic membrane domains only.
