Expression of three expansin genes during development and maturation of Kyoho grape berries
Expression of three expansin genes during development and maturation of Kyoho grape berries
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DOI：
10.1016/j.jplph.2006.07.017
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发表时间：
2007-12-01
影响因子：
4.3
通讯作者：
Kobayashi, Shozo
中科院分区：
文献类型：
--
作者：
Ishimaru, Megumi;Smith, David L.;Kobayashi, Shozo
Expansins是位于细胞壁的蛋白质，在体外以pH依赖性方式诱导分离的植物细胞壁的松动，但不表现出可检测到的水解酶或转糖基酶活性。从巨峰葡萄成熟浆果的cDNA文库中分离出三个推定的扩展蛋白cDNA，Vlexp1，Vlexp2和Vlexp3。在整个浆果发育过程中分析了3个基因的表达谱。Vlexp3转录物的积累与浆果软化密切相关，该基因的表达在变熟前检测到，并在变熟时显著增加（浆果软化开始）。Vlexp3的表达是浆果特异性的。Vlexp1和Vlexp2的mRNA积累开始在扩展阶段的浆果发展和成熟过程中两个基因的表达增加。Vlexp1和Vlexp2 mRNA在叶、卷须和花组织中检测到，Vlexp2 mRNA在根和种子组织中检测到。这些发现表明，这三个expansin基因与细胞分裂或扩展和浆果成熟。Vlexp3，特别是，最有可能发挥死记硬背在葡萄浆果软化在veraison。(C)2006年Elsevier GmbH。All rights reserved.
Expansins are cell-wall-localized proteins that induce Loosening of isolated plant cell walls in vitro in a pH-dependent manner, but exhibit no detectable hydrolase or transglycosylase activity. Three putative expansin cDNAs, Vlexp1, Vlexp2, and Vlexp3 were isolated from a cDNA library made from mature berries of the Kyoho grape. Expression profiles of the 3 genes were analyzed throughout berry development. Accumulation of the Vlexp3 transcript was closely correlated with berry softening, and expression of this gene was detected before veraison and markedly increased at veraison (onset of berry softening). Expression of Vlexp3 was berry-specific. Vlexp1 and Vlexp2 mRNA accumulation began during the expansion stage of berry development and expression increased for both genes during ripening. Vlexp1 and Vlexp2 mRNA was detected in Leaf, tendril and flower tissues and Vlexp2 mRNA was additionally detected in root and seed tissues. These findings suggest that the three expansin genes are associated with cell division or expansion and berry ripening. Vlexp3, in particular, is most likely to play a rote in grape berry softening at veraison. (C) 2006 Elsevier GmbH. All rights reserved.