Identification of endothelial cells and progenitor cell subsets in human peripheral blood.
Identification of endothelial cells and progenitor cell subsets in human peripheral blood.
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DOI：
10.1002/0471142956.cy0933s52
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Case, Jamie
中科院分区：
文献类型：
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作者：
Estes, Myka L;Mund, Julie A;Case, Jamie
由于内皮祖细胞 (EPC) 在血管生成和血管生成中发挥作用，因此通过流式细胞术对人外周血中的循环细胞亚群进行检测可用作确定心血管疾病风险和肿瘤对化疗的反应性的生物标志物。尽管多色流式细胞术 (PFC) 的分析取得了进展，但仍常规使用传统方法来计数和分离 EPC，这导致了临床研究中的不同结果、潜在的细胞错误识别，以及因此对所谓的 EPC 如何发挥作用缺乏合理的生物学解释。在此，提供了可重复的 PFC 方案来识别具有增殖潜力的罕见循环内皮集落形成细胞 (ECFC) 以及循环祖细胞 (CPC) 群体，其中比率分析可区分健康群体和疾病群体。总之，描述了一种可靠的 PFC 方案，可用于研究人类造血和内皮元件在脉管系统生长和维持中的作用。
An assay for circulating cell subsets in human peripheral blood by flow cytometry is used as a biomarker to determine cardiovascular disease risk and tumor responsiveness to chemotherapy since endothelial progenitor cells (EPCs) function in vasculogenesis and angiogenesis. Despite analytical advances in polychromatic flow cytometry (PFC), conventional approaches are routinely utilized to enumerate and isolate EPCs, which has led to varied results in clinical studies, potential cellular misidentification, and thus a lack of a plausible biological explanation for how purported EPCs function. Herein, a reproducible PFC protocol is provided to identify a rare circulating endothelial colony-forming cell (ECFC) with proliferative potential, along with a population of circulating progenitor cells (CPCs) in which the ratio analysis distinguishes between healthy and disease populations. In sum, a reliable PFC protocol, which can be used to investigate the roles of human hematopoietic and endothelial elements in the growth and maintenance of the vasculature, is described.