Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
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中性粒细胞胞外陷阱的循环水平并不是评估抗中性粒细胞胞质抗体相关性血管炎疾病活动性的有用生物标志物
DOI：
10.1371/journal.pone.0148197
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhao MH
中科院分区：
文献类型：
--
作者：
Wang H;Sha LL;Ma TT;Zhang LX;Chen M;Zhao MH
抗神经元胞质抗体（ANCA）相关性血管炎（AAV）是一组危及生命的疾病，经常影响肾脏。本研究调查了循环中性粒细胞胞外陷阱（NETs）水平是否与AAV的疾病活动相关。我们收集了34例活动期AV患者和62例缓解期AV患者的血清样本。使用Quant-iT PicoGreen测定法定量血清中的无细胞DNA。NET相关的MPO-DNA复合物，瓜氨酸化组蛋白H3-DNA（cit-H3-DNA）复合物和脱氧核糖核酸酶I（DNase I）的浓度使用ELISA定量。采用放射状酶扩散法定量测定DNA酶I的活性。分析了NET循环水平与临床病理参数之间的相关性。活动性AAV患者血清NETs水平显著高于健康对照组，且细胞游离DNA水平与C反应蛋白（CRP）水平相关。而MPO-DNA复合物或cit-H3-DNA复合物水平与CRP之间无相关性。此外，NETs水平与初始血清肌酐、估计肾小球滤过率（eGFR）、新月体形成或伯明翰血管炎活动评分（BVAS）之间无显著相关性。活动期和缓解期患者血清游离DNA和MPO-DNA复合物水平无显著性差异。总之，NET的循环水平不能用作评估AAV患者中疾病活动的生物标志物。
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of life-threatening disorders, and frequently affects the kidneys. This study investigated whether the circulating neutrophil extracellular traps (NETs) levels were associated with disease activity of AAV. We collected serum samples from 34 patients with AAV in active stage and 62 patients with AAV in remission. Cell free DNA in serum was quantified using the Quant-iT PicoGreen assay. NETs associated MPO-DNA complexes, citrullinated-histone H3-DNA (cit-H3-DNA) complexes and the concentration of deoxyribonuclease I (DNase I) were quantified using ELISA. The activity of DNase I was quantified using radial enzyme-diffusion method. Associations between circulating levels of NETs with clinico-pathological parameters were analyzed. Serum levels of NETs in active AAV patients were significantly higher than those in healthy controls, and the level of cell free DNA correlated with C-reactive protein (CRP). However, no correlation was found between MPO-DNA complexes or cit-H3-DNA complexes level and CRP. Also there was no significant correlation between NETs level and initial serum creatinine, estimated glomerular filtration rate (eGFR), crescents formation or Birmingham Vasculitis Activity Score (BVAS). Furthermore, there was no significant difference of serum levels of cell free DNA or MPO-DNA complexes between active stage and remission of AAV. In conclusion, circulating levels of NETs cannot be used as a biomarker to assess disease activity in AAV patients.