FUNCTIONAL-SIGNIFICANCE OF CUMULUS EXPANSION IN THE MOUSE - ROLES FOR THE PREOVULATORY SYNTHESIS OF HYALURONIC-ACID WITHIN THE CUMULUS MASS
FUNCTIONAL-SIGNIFICANCE OF CUMULUS EXPANSION IN THE MOUSE - ROLES FOR THE PREOVULATORY SYNTHESIS OF HYALURONIC-ACID WITHIN THE CUMULUS MASS
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DOI：
10.1002/mrd.1080340114
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发表时间：
1993-01-01
影响因子：
2.5
通讯作者：
LARSEN, WJ
中科院分区：
文献类型：
--
作者：
CHEN, L;RUSSELL, PT;LARSEN, WJ
促性腺激素刺激的小鼠卵丘卵母细胞复合体（COC）在体外的扩张，用定量摄像方法测量，是可比的，观察到发生在体内时，培养基中补充有猪促卵泡激素（FSH），10%胎牛血清（FBS），和2.5 mM葡萄糖胺或最佳浓度的谷氨酰胺和葡萄糖。在不存在葡糖胺的情况下，COCs在体外的体积膨胀从未超过其存在时发生的体积膨胀的25%。添加6-重氮-5-氧代-1-正亮氨酸（DON），一种葡萄糖胺合成的抑制剂添加到培养基中谷氨酰胺和葡萄糖，完全抑制卵丘扩展在体外。利用该系统研究了积云扩展与受精率之间的关系，以及培养中受精力的维持。成功受精（由发育到2-细胞阶段确定）与扩展的卵丘质量的数量和质量相关，相反，卵丘的自发损失或机械去除与在培养基中额外孵育后的受精能力损失相关。此外，腹膜内注射DON可抑制完整卵泡内的卵丘扩张并抑制排卵。
Gonadotropin-stimulated expansion of the mouse cumulus oocyte complex (COC) in vitro, measured with a quantitative videographic method, is comparable to that observed to occur in vivo when medium is supplemented with porcine follicle stimulating hormone (FSH), 10% fetal bovine serum (FBS), and 2.5 mM glucosamine or optimal concentrations of glutamine and glucose. In the absence of glucosamine, the volumetric expansion of COCs in vitro is never more than 25% of that occurring in its presence. The addition of 6-diazo-5-oxo-1-norleucine (DON), an inhibitor of glucosamine synthesis to medium supplemented with glutamine and glucose, completely inhibits cumulus expansion in vitro. This system was utilized to examine the relationship between cumulus expansion and fertilization rates, and the maintenance of fertilizability in culture. Successful fertilization (as determined by development to the 2-cell stage) was correlated with the quantity and quality of the expanded cumulus mass, and conversely, the spontaneous loss or mechanical removal of the cumulus was correlated with a loss of fertilizability following additional incubation in culture medium. In addition, the i.p. injection of DON inhibited cumulus expansion within the intact follicle and suppressed ovulation.