An ABC Transporter Is Involved in the Silicon-Induced Formation of Casparian Bands in the Exodermis of Rice.
An ABC Transporter Is Involved in the Silicon-Induced Formation of Casparian Bands in the Exodermis of Rice.
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DOI：
10.3389/fpls.2017.00671
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发表时间：
2017
影响因子：
5.6
通讯作者：
Schenk MK
中科院分区：
文献类型：
--
作者：
Hinrichs M;Fleck AT;Biedermann E;Ngo NS;Schreiber L;Schenk MK
硅（Si）促进水稻凯氏带（CB）的形成，并减少径向氧损失（ROL）。进一步的转录组学方法揭示了参与Si诱导的CB形成的几个候选基因，如ATP结合盒（ABC）转运蛋白、III类过氧化物酶、连接酶和转移酶。通过过表达（OE）和敲除（KO）突变体对这些基因的研究揭示了ABC转运蛋白（OsABCG 25）对外皮中CB形成的贡献，这也反映在其他OsABCG 25在Si促进的与苯丙烷途径相关的CB基因的形成中的表达，如苯丙氨酸-氨-裂解酶、二酰基甘油O-酰基转移酶和4-香豆酸-CoA连接酶。突变体和Si供应的差异CB发展也影响了外皮层的屏障功能。ABC转运蛋白的OE和Si供应降低了来自根的ROL和Fe吸收。对于KO突变体，未观察到对ROL和Fe摄取的影响。所提出的研究证实了OsABCG 25在Si促进的CB形成及其阻挡功能中的影响。
Silicon (Si) promotes the formation of Casparian bands (CB) in rice and reduces radial oxygen loss (ROL). Further transcriptomic approaches revealed several candidate genes involved in the Si-induced formation of CB such as ATP binding cassette (ABC) transporter, Class III peroxidases, ligases and transferases. Investigation of these genes by means of overexpression (OE) and knockout (KO) mutants revealed the contribution of the ABC transporter (OsABCG25) to CB formation in the exodermis, which was also reflected in the expression of other OsABCG25 in the Si-promoted formation of CB genes related to the phenylpropanoid pathway, such as phenylalanine-ammonia-lyase, diacylglycerol O-acyltransferase and 4-coumarate-CoA ligase. Differential CB development in mutants and Si supply also affected the barrier function of the exodermis. OE of the ABC transporter and Si supply reduced the ROL from roots and Fe uptake. No effect on ROL and Fe uptake could be observed for the KO mutant. The presented research confirms the impact of the OsABCG25 in the Si-promoted formation of CB and its barrier functions.