Influenza activity in Cambodia during 2006-2008.
Influenza activity in Cambodia during 2006-2008.
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DOI：
10.1186/1471-2334-9-168
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发表时间：
2009-10-15
影响因子：
3.7
通讯作者：
Buchy P
中科院分区：
文献类型：
--
作者：
Mardy S;Ly S;Heng S;Vong S;Huch C;Nora C;Asgari N;Miller M;Bergeri I;Rehmet S;Veasna D;Zhou W;Kasai T;Touch S;Buchy P
关于柬埔寨人口中流行性感冒疾病的信息很少。为了更好地了解柬埔寨的流感动态，2006年8月成立了柬埔寨国家流感中心。为了持续监测流感活动，2006年在五个地点建立了以医院为基础的ILI（流感样疾病）哨点监测系统，每周报告和抽样计划。此外，在2个地点建立了急性下呼吸道感染（ALRI）病例的医院监测。哨点每周收集符合病例定义的ILI患者的流行病学数据，并每周从确定数量的病例中采集鼻咽标本。这些样本在金边巴斯德研究所病毒学部门进行检测。从每个样本中提取病毒RNA并通过多重RT-PCR扩增，同时检测甲型流感和乙型流感病毒。然后用血凝抑制试验分析甲型流感病毒亚型。用同样的方法测试由ALRI系统收集的样品。2006年至2008年，流感流行主要发生在6月至12月，2008年的数据显示，10月出现明显的季节性高峰。柬埔寨的流感活动发生在6月至12月的雨季，并在凉爽季节（通常从12月延长至2月）之前结束。虽然柬埔寨是一个热带国家，地理位置位于北半球，但流感活动具有南半球传播模式。结合对流行毒株的抗原分析，现在有可能为柬埔寨提供更好的流感疫苗接种建议。
There is little information about influenza disease among the Cambodian population. To better understand the dynamics of influenza in Cambodia, the Cambodian National Influenza Center (NIC) was established in August 2006. To continuously monitor influenza activity, a hospital based sentinel surveillance system for ILI (influenza like illness) with a weekly reporting and sampling scheme was established in five sites in 2006. In addition, hospital based surveillance of acute lower respiratory infection (ALRI) cases was established in 2 sites. The sentinel sites collect weekly epidemiological data on ILI patients fulfilling the case definition, and take naso-pharyngeal specimens from a defined number of cases per week. The samples are tested in the Virology Unit at the Institut Pasteur in Phnom Penh. From each sample viral RNA was extracted and amplified by a multiplex RT-PCR detecting simultaneously influenza A and influenza B virus. Influenza A viruses were then subtyped and analyzed by hemagglutination inhibition assay. Samples collected by the ALRI system were tested with the same approach. From 2006 to 2008, influenza circulation was observed mainly from June to December, with a clear seasonal peak in October shown in the data from 2008. Influenza activity in Cambodia occurred during the rainy season, from June to December, and ended before the cool season (extending usually from December to February). Although Cambodia is a tropical country geographically located in the northern hemisphere, influenza activity has a southern hemisphere transmission pattern. Together with the antigenic analysis of the circulating strains, it is now possible to give better influenza vaccination recommendation for Cambodia.