Effect of protein adsorption on the transport characteristics of asymmetric ultrafiltration membranes.
Effect of protein adsorption on the transport characteristics of asymmetric ultrafiltration membranes.
复制标题
蛋白质吸附对不对称超滤膜传输特性的影响。
DOI：
10.1021/bp00018a013
复制
发表时间：
1992
影响因子：
2.9
通讯作者：
Zydney,AL
中科院分区：
文献类型：
--
作者：
Mochizuki,S;Zydney,AL
采用凝胶渗透色谱法，以多分散葡聚糖为吸附剂，研究了牛血清白蛋白吸附对非对称聚醚砜超滤膜传递性能的影响。实际的葡聚糖筛分系数进行了评价，从观察到的筛分数据的清洁和预吸附膜使用停滞膜模型。实际葡聚糖筛分系数的通量依赖性被用来评估使用经典膜转运理论的不同分子量葡聚糖的对流（即筛分）和扩散转运的固有膜阻碍因子。蛋白质吸附导致葡聚糖筛分和扩散的减少，减少的幅度是葡聚糖分子量和孔径的函数。吸附的比孔面积和膜的孔隙率的影响，然后使用最近的模型通过不对称超滤膜的溶质传输。这些数据表明，蛋白质吸附优先发生在largermembrane孔，导致更大的减少溶质筛分相比，膜的水力渗透性和孔隙率比将预测的基础上，无论是一个简单的孔堵塞或孔收缩模型。
The effects of bovine serum albumin adsorption on the transport characteristics of asymmetric poly (ether sulfone) ultrafiltration membranes were determined using polydisperse dextrans with gel permeation chromatograhy. Actual dextran sieving coefficients were evaluated from observed sieving data for both the clean and preadsorbed membranes using a stagnant film model. The flux dependence of the actual dextran sieving coefficients was used to evaluate the intrinsic membrane hindrance factors for convective (ie, sieving) and diffusivetransport for the different molecular weight dextrans using classical membrane transport theory. Protein adsorption caused a reduction in both dextran sieving and diffusion, with the magnitude of the reduction a function of the dextran molecular weight andpore size. The effects of adsorption on the specific pore area and the membrane porosity were then determined using a recent model for solute transport through asymmetric ultrafiltration membranes. The data indicate that protein adsorption occurs preferentially in the largermembrane pores, causing a greater reduction in solute sieving compared to the membrane hydraulic permeability and porosity than would be predicted on the basis of either a simple pore blockage or pore constriction model.