Growth and development of Toxoplasma gondii in human neurons and astrocytes
Growth and development of Toxoplasma gondii in human neurons and astrocytes
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DOI：
10.1097/00005072-199611000-00006
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发表时间：
1996-11-01
影响因子：
3.2
通讯作者：
Chiu, FC
中科院分区：
文献类型：
--
作者：
Halonen, SK;Lyman, WD;Chiu, FC
弓形虫是影响艾滋病患者中枢神经系统最常见的机会性感染之一。疾病是由于大脑中的潜伏感染重新激活而导致严重的坏死性脑炎。在本研究中，我们用弓形虫感染人胎脑原代培养物，并研究了该培养系统中活跃期和潜伏期的行为。我们发现，弓形虫的活跃期(速殖子)可以感染星形胶质细胞和神经元。然而，星形胶质细胞被感染的比例高于神经元。此外，星形胶质细胞比神经元支持更多的弓形虫复制。星形胶质细胞和神经元也支持在潜伏感染中发现的囊肿期。这些数据表明，星形胶质细胞是支持弓形虫在脑内的大部分复制的宿主细胞，但在潜伏感染中，这两种宿主细胞类型可能都能够支持包囊阶段。然而，有证据表明，在星形胶质细胞中形成的囊肿可能不同于神经元囊肿。这些发现可能与艾滋病患者潜在的弓形虫感染重新激活有关。
Toxoplasma gondii (T. gondii) is one of the most common opportunistic infections affecting the central nervous system (CNS) in AIDS patients. Disease results from a reactivation of a latent infection in the brain resulting in a severe and necrotizing encephalitis. In this study we infected a primary culture from human fetal brain with T. gondii and studied the behavior of both the active and latent stages in this culture system. We found that the active (tachyzoite) stage of T. gondii can infect both astrocytes and neurons. However, a higher percentage of astrocytes were infected than neurons. Additionally astrocytes were found to support more replication of T. gondii than did neurons. Both astrocytes and neurons also supported the cyst stage, found in the latent infections. These data indicate that astrocytes are the host cells supporting most of the replication of T. gondii in the brain in reactivated infections, but both host cell types may be able to support the cyst stage in latent infections. However, evidence indicates that cysts formed in astrocytes may be distinct from neuronal cysts. These findings may have relevance to reactivation of latent T. gondii infections in AIDS patients.