Dissection of the genetic basis of oil content in Chinese peanut cultivars through association mapping
Dissection of the genetic basis of oil content in Chinese peanut cultivars through association mapping
复制标题
通过关联作图解析中国花生品种含油量的遗传基础
DOI：
10.1186/s12863-020-00863-1
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发表时间：
2020-06-08
期刊：
影响因子：
2.9
通讯作者：
Jiang, Huifang
中科院分区：
文献类型：
--
作者：
Liu, Nian;Huang, Li;Jiang, Huifang
花生是世界上主要的植物油来源之一，提高含油量是世界上许多花生育种计划的主要目标。紧密连锁的标记是需要更快地发展高油含量花生品种，通过基因组辅助育种（GAB），和关联作图是有前途的方法之一，发现这样的关联markers. ResultsA关联作图面板组成的292个花生品种广泛分布在中国的表型油含量和基因型与583个多态性SSR标记。这些标记共扩增出3663个等位基因，平均每个位点扩增出6.28个等位基因。结构，系统发育关系和主成分分析（PCA）表明两个亚组主要区分基于地理区域。全基因组关联分析确定了12个相关标记，包括一个（AGGS 1014_2）高度稳定的关联控制高达9.94%的表型方差解释（PVE）在多个环境。有趣的是，12个相关标记的有利等位基因的频率显示出地理差异。两个相关标记结果表明，PVE值为6.90-9.94%的AGGS 1014_2和AHGS 0798在独立的RIL群体中表现出较高的含油量，在育种过程中具有一定的选择作用。含油量选育花生品种。
BackgroundPeanut is one of the primary sources for vegetable oil worldwide, and enhancing oil content is the main objective in several peanut breeding programs of the world. Tightly linked markers are required for faster development of high oil content peanut varieties through genomics-assisted breeding (GAB), and association mapping is one of the promising approaches for discovery of such associated markers.ResultsAn association mapping panel consisting of 292 peanut varieties extensively distributed in China was phenotyped for oil content and genotyped with 583 polymorphic SSR markers. These markers amplified 3663 alleles with an average of 6.28 alleles per locus. The structure, phylogenetic relationship, and principal component analysis (PCA) indicated two subgroups majorly differentiating based on geographic regions. Genome-wide association analysis identified 12 associated markers including one (AGGS1014_2) highly stable association controlling up to 9.94% phenotypic variance explained (PVE) across multiple environments. Interestingly, the frequency of the favorable alleles for 12 associated markers showed a geographic difference. Two associated markers (AGGS1014_2 and AHGS0798) with 6.90–9.94% PVE were verified to enhance oil content in an independent RIL population and also indicated selection during the breeding program.ConclusionThis study provided insights into the genetic basis of oil content in peanut and verified highly associated two SSR markers to facilitate marker-assisted selection for developing high-oil content breeding peanut varieties.