Follicular-phase ovarian follicular fluid and plasma cytokine profiling of natural cycle in vitro fertilization patients
Follicular-phase ovarian follicular fluid and plasma cytokine profiling of natural cycle in vitro fertilization patients
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DOI：
10.1016/j.fertnstert.2014.04.032
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发表时间：
2014-08-01
影响因子：
6.7
通讯作者：
Sharma, Vinay
中科院分区：
文献类型：
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作者：
Baskind, N. Ellissa;Orsi, Nicolas M.;Sharma, Vinay
目的：在自然周期卵泡和排卵期的不同时间点描述卵泡液（FF）和全身细胞因子的特征。设计：连续两个周期的观察性临床研究。设置：医院为基础的体外受精程序。患者：10名接受改良自然周期体外受精（MNC-IVF）的妇女。干预：血浆和卵泡液（FF）收集。主要结果测量：在两个连续周期中，通过液相多重免疫测定法测定每例患者个体卵泡和血浆中的40种FF细胞因子浓度：1）跟踪周期-卵泡中期或黄体峰; 2）治疗周期-排卵期（在MNC-IVF时）。人口统计学，周期和细胞因子的数据进行了比较，使用卡方，配对分数t检验，或Wilcoxon签署的秩tests. Results（S）：波动在各种FF细胞因子是明显的卵泡期：白细胞介素（IL）6和IL-8的水平较高的排卵期样本，IL-1受体拮抗剂和血管内皮生长因子升高，在周期的早期。黄体峰特征与排卵期样本中发现的相似。相反，循环细胞因子浓度更稳定，在follicular phase. Conclusions（S）：这些研究结果提出了广泛的生理参考档案FF细胞因子与窦folliculogenesis和突出划分的系统和卵巢内细胞因子网络在自然周期。（（C）2014年由美国生殖医学协会。
Objective: To characterize follicular fluid (FF) and systemic cytokine profiles at various time points during the natural-cycle follicular and periovulatory phases.Design: Observational clinical study across two consecutive cycles.Setting: Hospital-based in vitro fertilization program.Patient(s): Ten women undergoing modified natural-cycle in vitro fertilization (MNC-IVF).Intervention(s): Plasma and follicular fluid (FF) collection.Main Outcome Measure(s): Forty FF cytokine concentrations from individual follicles and plasma from each patient were determined by fluid-phase multiplex immunoassay in two consecutive cycles: 1) tracking cycle-midfollicular or luteal surge; and 2) treatment cycle-periovulatory (at the time of MNC-IVF). Demographic, cycle, and cytokine data were compared with the use of chi-square, paired-scores t test, or Wilcoxon signed ranks tests.Result(s): Fluctuations in various FF cytokines were evident during the follicular phase: Levels of interleukin (IL) 6 and IL-8 were higher in periovulatory samples, and IL-1 receptor antagonist and vascular endothelial growth factor were elevated earlier in the cycle. Luteal surge profiles were similar to those found in periovulatory samples. Conversely, circulatory cytokine concentrations were more stable during the follicular phase.Conclusion(s): These findings present an extensive physiologic reference profile of FF cytokines associated with antral folliculogenesis and highlight the compartmentalization of systemic and intraovarian cytokine networks in natural cycles. ((C)2014 by American Society for Reproductive Medicine.)