Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid: two gene products involved in plant tumorigenesis.
Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid: two gene products involved in plant tumorigenesis.
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pTiA6NC 章鱼 Ti 质粒的 tms 基因的核苷酸序列：参与植物肿瘤发生的两个基因产物。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
M. Gordon
中科院分区：
文献类型：
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作者：
H. Klee;A. Montoya;F. Horodyski;C. Lichtenstein;D. Garfinkel;S. Fuller;C. Flores;J. Peschon;E. Nester;M. Gordon
已经确定了来自pTiA 6 NC的肿瘤形态学基因座tms的核苷酸序列。序列分析表明，由该基因座编码的两个多聚腺苷酸化转录物中的每一个都含有开放阅读框;预测的转录物1基因产物的分子大小为83，769道尔顿，预测的转录物2基因产物的分子大小为49，588道尔顿。转录本2 RNA的精确起始和终止位置已经用S1核酸酶作图。已经构建了几个插入突变。其中之一将转录物2启动子定位在转录起始的5'端的72个碱基对内。显着的同源性之间观察到的蛋白质编码的转录本1和腺嘌呤结合区的对羟基苯甲酸羟化酶从荧光假单胞菌，这表明转录本1蛋白结合腺嘌呤作为底物或辅因子。
The nucleotide sequence of the tumor morphology locus, tms, from pTiA6NC has been determined. The sequence analysis indicates that each of two polyadenylylated transcripts encoded by this locus contains an open reading frame; the predicted transcript 1 gene product has a molecular size of 83,769 daltons, and the predicted transcript 2 gene product, of 49,588 daltons. The precise start and stop positions of the transcript 2 RNA have been mapped with S1 nuclease. Several insertion mutations have been constructed. One of these localizes the transcript 2 promoter within the 72 base pairs 5' to transcription initiation. Significant homology was observed between the protein encoded by transcript 1 and the adenine binding region of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens, suggesting that the transcript 1 protein binds adenine either as substrate or cofactor.