The simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2 and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts.
The simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2 and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts.
复制标题
同时使用两种前列腺素放射免疫测定法，采用两种不同特异性的抗血清。
DOI：
10.1016/0090-6980(76)90060-5
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
W. Stylos
中科院分区：
文献类型：
--
作者：
E. Ritzi;W. Stylos
用3种不同的方法评价了前列腺素E1、E2和F1 α在BALB/c 3 T3和SV 3 T3细胞培养物中的相对稳定性。首先，孵育和提取PGF 1 α和PGE 1后乙酸乙酯相中氚的总回收率表明PGF 1 α（88.8%）的稳定性高于PGE 1（65.9%）。第二，孵育，提取，氚标记的PG薄层色谱分析显示减少高达23%的PGE区孵育后的3 H-PGE 1。随着孵育时间的增加，PGE区的减少伴随着PGA样化合物的增加。3 H-PGF 1 α表现出更高的稳定性，在PGF区中的氚回收率大于90%。评估培养物中PG稳定性的第三种方法是通过放射免疫测定法（RIA）比较单个PG的产生。通过RIA获得的数据表明PGA和PGB的增加滞后，直到注意到PGE的初始上升，这表明PGA和PGB可能是由PGE产生的次级产物，其在培养中仅表现出部分稳定性。通过采用两种RIA，一种用于总PGE，一种用于PGA和PGB，由于PG的不稳定性，复合测定PG [E+（A+ B）]可用于提供更有意义的PG产生测定。另一方面，单个测定有助于评估细胞培养物中PGE的稳定性。
The relative stability of Prostaglandins (PGs) E1, E2 and F1alpha in cultures of BALB/c 3T3 and SV3T3 cells has been evaluated using 3 different approaches. First, total recovery of tritium in the ethyl acetate phase following incubation and extraction of PGF1alpha and PGE1 demonstrated greater stability for PGF1alpha (88.8%) than PGE1 (65.9%). Second, analysis of incubated, extracted, tritiated PGs by thin layer chromatography revealed decreases of up to 23% in the PGE zone following incubation of 3H-PGE1. With increasing time of incubation, decreases in the PGE zone were accompanied by increase in PGA-like compounds. 3H-PGF1alpha demonstrated greater stability, having greater than 90% recovery of the tritium in the PGF zone. A third approach to the assessment of PG stability in culture was the comparison of the production of individual PGs by radioimmunoassay (RIA). The data obtained by RIA indicated a lag in the increase of PGA and PGB, until an initial rise in PGE was noted, suggesting that PGA and PGB may be secondary products arising from PGE which exhibits only partial stability in culture. By employing two RIAs, one for total PGE and one for PGA and PGB, the composite determination PG [E+(A+ B)] can be used to provide a more meaningful determination of PG production because of the instability of the PGs. On the other hand, individual determinations are helpful in assessing the stability of PGEs in cell cultures.