Transgenic stem cells in Hydra reveal an early evolutionary origin for key elements controlling self-renewal and differentiation
Transgenic stem cells in Hydra reveal an early evolutionary origin for key elements controlling self-renewal and differentiation
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DOI：
10.1016/j.ydbio.2007.06.013
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发表时间：
2007-09-01
影响因子：
2.7
通讯作者：
Bosch, Thomas C. G.
中科院分区：
文献类型：
--
作者：
Khalturin, Konstantin;Anton-Erxleben, Friederike;Bosch, Thomas C. G.
在进化之初，人们对生物体中的干细胞知之甚少。为了表征在基础后生动物中控制干细胞的调控事件，我们产生了在间质干细胞谱系中选择性表达EGFP的转基因细胞。使用它们，我们在本地环境中实时可视化了干细胞和前体的迁移。我们证明间质细胞对来自细胞环境的信号做出反应，而WRIT和Notch通路是这一过程中的关键角色。此外，通过分析息肉在其间质细胞中过表达多梳蛋白HyEED，我们为染色质修饰在终末分化中的作用提供了体内证据。这些发现首次揭示了在比拉特里亚祖先中涉及干细胞分化的信号通路；它们表明，控制干细胞行为的机制是基于整个动物界保守的成分。(C)2007 Elsevier Inc.保留所有权利。
Little is known about stem cells in organisms at the beginning of evolution. To characterize the regulatory events that control stem cells in the basal metazoan Hydra, we have generated transgenics which express eGFP selectively in the interstitial stem cell lineage. Using them we visualized stem cell and precursor migration in real-time in the context of the native environment. We demonstrate that interstitial cells respond to signals from the cellular environment, and that Writ and Notch pathways are key players in this process. Furthermore, by analyzing polyps which overexpress the Polycomb protein HyEED in their interstitial cells, we provide in vivo evidence for a role of chromatin modification in terminal differentiation. These findings for the first time uncover insights into signalling pathways involved in stem cell differentiation in the Bilaterian ancestor; they demonstrate that mechanisms controlling stem cell behaviour are based on components which are conserved throughout the animal kingdom. (c) 2007 Elsevier Inc. All rights reserved.