A novel role for DNA photolyase: binding to DNA damaged by drugs is associated with enhanced cytotoxicity in Saccharomyces cerevisiae.
A novel role for DNA photolyase: binding to DNA damaged by drugs is associated with enhanced cytotoxicity in Saccharomyces cerevisiae.
复制标题
DNA 光裂合酶的一个新作用：与药物损伤的 DNA 结合与酿酒酵母细胞毒性的增强有关。
DOI：
10.1128/mcb.14.12.8071-8077.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Chu,G
中科院分区：
文献类型：
--
作者：
Fox,ME;Feldman,BJ;Chu,G
DNA photolyase binds to and repairs cyclobutane pyrimidine dimers induced by UY radiation. Here we demonstrate that ih the yeastSaccharomyces cerevisiae,photolyase also binds to DNA damaged by the anticancer drugscis-diamminedichloroplatinum (cis-DDP) and nitrogen mustard (HN2) and by the alkylating agentN-methyl-N′-nitro-N-nitrosoguanidine (MNNG). Surprisingly, mutations in photolyase were associated with resistance of yeast cells tocis-DDP, MNNG, 4-nitroquinoline oxide (4NQO), and HN2. Transformation of yeast photolyase mutants with the photolyase gene increased sensitivity to these agents. Thus, while the binding of photolyase to DNA damaged by UV radiation aids survival of the cell, binding to DNA damaged by other agents may interfere with cell survival, perhaps by making the lesions inaccessible to the nucleotide excision repair system.
