The Fungal Endophyte Epicoccum dendrobii as a Potential Biocontrol Agent Against Colletotrichum gloeosporioides
The Fungal Endophyte Epicoccum dendrobii as a Potential Biocontrol Agent Against Colletotrichum gloeosporioides
复制标题
真菌内生菌 Epicocum dendrobii 作为对抗胶孢炭疽病的潜在生物防治剂
DOI：
10.1094/phyto-05-20-0170-r
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发表时间：
2021-02-01
期刊：
影响因子：
3.2
通讯作者：
Huang, Lin
中科院分区：
文献类型：
--
作者：
Bian, Jin-Yue;Fang, Yu-Lan;Huang, Lin
炭疽病是杉木上最严重的真菌病害之一。从杉木幼嫩健康分支中分离到8株内生真菌，并进行了离体筛选。一个分离物，命名为SMEL1，随后确定为Epicoccum dendrobii的形态和系统发育分析的基础上，抑制菌丝生长的炭疽gloeosporioides双培养板。此外，E.石斛代谢产物显著降低了胶孢炭疽菌的生物量。E.石斛能通过气孔细胞进入寄主植物的内部组织。E.石斛内生菌显著抑制孢子萌发和附着胞形成，这至少部分解释了为什么内生菌显著抑制炭疽菌引起的各种寄主植物的病变发展。进一步证实了从E.石斛，使用乙酸乙酯作为有机溶剂。据我们所知，这是E.作为抗真菌植物病原体的潜在生物防治剂。
Anthracnose caused by Colletotrichum gloeosporioides is one of most serious fungal diseases on Chinese fir (Cunninghamia lanceolata). Eight fungal endophytes were isolated from a young heathy branch of Chinese fir and screened against the pathogen in vitro. One isolate, designated as SMEL1 and subsequently identified as Epicoccum dendrobii based on morphological and phylogenetic analyses, suppressed mycelial growth of Colletotrichum gloeosporioides on dual-culture plates. Additionally, E. dendrobii metabolites significantly decreased the biomass of Colletotrichum gloeosporioides. E. dendrobii was able to enter the internal tissues of the host plant via stomatal cells. Metabolites of E. dendrobii significantly inhibited conidial germination and appressorium formation, which at least partly explained why the endophyte significantly inhibited lesion development caused by Colletotrichum gloeosporioides on various host plants. We further confirmed that some components with antifungal activity could be extracted from E. dendrobii using ethyl acetate as an organic solvent. To our knowledge, this is the first report of E. dendrobii as a potential biocontrol agent against a fungal phytopathogen.