PHARMACOLOGIC CHARACTERIZATION OF NICOTINE-INDUCED CONDITIONED PLACE PREFERENCE
PHARMACOLOGIC CHARACTERIZATION OF NICOTINE-INDUCED CONDITIONED PLACE PREFERENCE
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DOI：
10.1016/0091-3057(85)90384-3
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
IWAMOTO, ET
中科院分区：
文献类型：
--
作者：
FUDALA, PJ;TEOH, KW;IWAMOTO, ET
大鼠接受s.c.在条件性位置偏爱（CPP）范例中，在条件性阶段之前立即注射尼古丁（0.1-1.2 mg/kg）或盐水（1.0 ml/kg）。将药物与3室位置偏好装置的非首选环境配对进行3次调节;生理盐水与首选环境配对。然后通过确定在15分钟测试期间在首选和非首选隔室中花费的时间比例来测试动物的位置偏好。使用为CPP范例开发的统计方法，获得尼古丁的奖励和厌恶效应的剂量-反应曲线，通过其改变先前确定的对照动物基线偏好的能力进行测量。在0.1-0.8 mg/kg剂量范围内，尼古丁的奖赏效应和厌恶效应与剂量呈线性相关（奖赏增加，厌恶减少）。在1.2 mg/kg给药水平下测量到奖励减少和厌恶增加。还使用CPP范例测试了盐酸美加明和溴化六甲双铵（分别为1.0 mg/kg碱或离子）。虽然单独给药时两种化合物都没有产生位置偏好，但在尼古丁条件反射前30分钟给药时，美加明确实阻断了0.8 mg/kg尼古丁的奖励作用。六甲双铵没有改变尼古丁诱导的强化。显然，尼古丁及其通过CPP测量的奖赏效应主要由中枢而不是外周事件介导。
Rats received s.c. injections of either nicotine (0.1-1.2 mg/kg) or saline (1.0 ml/kg) immediately prior to conditioning sessions in a conditioned place preference (CPP) paradigm. The drug was paired for 3 conditioning sessions with the non-preferred environment of a 3 compartment place preference apparatus; saline was paired with the preferred environment. The animals were then tested for place preference by determining the proportion of time spent in the preferred and non-preferred compartments during a 15 min test session. Using a statistical method developed for the CPP paradigm, dose-response curves were obtained for the rewarding and aversive effects of nicotine as measured by its ability to alter previously determined baseline preferences obtained from the control animals. Nicotine''s rewarding and aversive effects were linearly correlated with respect to dosage within the range of 0.1-0.8 mg/kg (reward increased and aversion decreased). A decrease in reward and an increase in aversion was measured at the 1.2 mg/kg treatment level. Mecamylamine hydrochloride and hexamethonium bromide (at 1.0 mg/kg of the base or ion, respectively) were also tested using the CPP paradigm. While neither compound produced place preferences when administered alone, mecamylamine did block the rewarding effects of 0.8 mg/kg of nicotine when administered 30 min prior to the nicotine conditioning sessions. Hexamethonium did not alter nicotine-induced reinforcement. Apparently, nicotine and its rewarding effects as measured by CPP are primarily mediated by central rather than peripheral events.