Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity.
Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity.
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德国蟑螂提取物中的过敏原含量和对一组新扩张的蟑螂过敏原的敏化曲线决定了IgE反应性的体外提取效力。
DOI：
10.1016/j.jaci.2018.07.036
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Pomés A
中科院分区：
文献类型：
--
作者：
Glesner J;Filep S;Vailes LD;Wünschmann S;Chapman MD;Birrueta G;Frazier A;Jeong KY;Schal C;Bacharier L;Beigelman A;Busse P;Schulten V;Sette A;Pomés A
蟑螂过敏原是城市哮喘患者ige介导致敏的重要原因。然而，用于诊断和免疫治疗的蟑螂提取物尚未标准化。测定非标准化德国蟑螂提取物中过敏原的含量以及对一组扩大的蟑螂过敏原的致敏水平，作为体外提取物的IgE反应性效力的决定因素。比较了12种德国蜚蠊提取物的过敏原含量和IgE反应活性。采用免疫分析法检测Bla g1、Bla g2和Bla g5。采用ImmunoCAP检测1、2、4、5、6、7、9、11组8个纯化重组过敏原的IgE抗体水平。采用IgE抗体结合抑制试验，对5名蟑螂过敏受试者进行体外效价（IC30）评估。过敏原水平变化很大。在高蟑螂致敏受试者（CAP-class≥3）中发现了3个新的主要过敏原（6、9和11组）。每个受试者的致敏特征是独特的，没有免疫优势过敏原。8种过敏原成分的IgE总和与蟑螂特异性IgE有较好的相关性（r = 0.88, p < 0.001）。体外效力在每个受试者的不同提取物之间以及每种提取物的受试者之间有所不同。德国蜚蠊提取物对IgE反应性的体外效力取决于致敏原含量和致敏原特异性IgE滴度。这些因素与选择用于免疫治疗的强效提取物以及设计和解释免疫治疗试验数据有关。非标准化蟑螂提取物中的过敏原含量和受试者的过敏原特异性致敏谱不同，并决定了体外提取物的IgE反应性效力。对于诊断和免疫治疗的提取物选择，应考虑这两个变量。
Cockroach allergens are an important cause of IgE-mediated sensitization in inner-city asthmatics. However, cockroach extracts used for diagnosis and immunotherapy are not standardized. To determine the allergen content of non-standardized German cockroach extracts and the levels of sensitization to an expanded set of cockroach allergens as determinants of in vitro extract potency for IgE reactivity. Twelve German cockroach extracts were compared for allergen content and for potency of IgE reactivity. Bla g 1, Bla g 2 and Bla g 5 were measured by immunoassays. IgE antibody levels to eight purified recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9 and 11 were measured by ImmunoCAP. IgE antibody binding inhibition assays were performed to assess extract in vitro potencies (IC30) relative to an arbitrarily selected reference extract in five cockroach allergic subjects. Allergen levels were highly variable. Three new major allergens (groups 6, 9 and 11), were identified among highly cockroach-sensitized subjects (CAP-class ≥ 3). Sensitization profiles were unique per subject, without immunodominant allergens. The sum of IgE to eight allergen components showed a good correlation with cockroach-specific IgE (r = 0.88; p < 0.001). In vitro potencies varied among different extracts per subject, and among subjects for each extract. The in vitro potency of German cockroach extracts for IgE reactivity depends on allergen content and allergen-specific IgE titers of the cockroach-allergic subject. These factors are relevant for selection of potent extracts to be used for immunotherapy and for the design and interpretation of data from immunotherapy trials. Allergen content in non-standardized cockroach extracts and subjects’ allergen-specific sensitization profiles vary and define the in vitro extract potency for IgE reactivity. Both variables should be considered regarding extract selection for diagnosis and immunotherapy.
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影响因子：
2.8
作者：
Jeong, Kyoung Yong;Kim, Chung-ryul;Yong, Tai-Soon
通讯作者：
Yong, Tai-Soon
影响因子：
14.2
作者：
Mueller, Geoffrey A.;Pedersen, Lars C.;Pomes, Anna
通讯作者：
Pomes, Anna
影响因子：
14.2
作者：
Kitzmueller, Claudia;Kalser, Julia;Bohle, Barbara
通讯作者：
Bohle, Barbara
影响因子：
14.2
作者：
Wood, Robert A.;Togias, Alkis;Wildfire, Jeremy;Visness, Cynthia M.;Matsui, Elizabeth C.;Gruchalla, Rebecca;Hershey, Gurjit;Liu, Andrew H.;O'Connor, George T.;Pongracic, Jacqueline A.;Zoratti, Edward;Little, Frederic;Granada, Mark;Kennedy, Suzanne;Durham, Stephen R.;Shamji, Mohamed H.;Busse, William W.
通讯作者：
Busse, William W.
影响因子：
158.5
作者：
Rosenstreich, DL;Eggleston, P;Malveaux, F
通讯作者：
Malveaux, F