MiCASA is a new method for quantifying cellular organization.
MiCASA is a new method for quantifying cellular organization.
复制标题
MiCASA是一种定量细胞组织的新方法。
DOI：
10.1038/ncomms15619
复制
发表时间：
2017-05-30
影响因子：
16.6
通讯作者：
Manley NR
中科院分区：
文献类型：
--
作者：
Sornborger A;Li J;Timmons C;Lupu F;Eggenschwiler J;Takahama Y;Manley NR
虽然存在许多用于识别和量化单个细胞类型的工具，但很少有方法可用于评估器官和组织中细胞类型之间的关系以及这些关系在衰老或疾病状态期间如何变化。我们提出了一种定量的方法来评估细胞的组织，使用小鼠胸腺作为测试器官。胸腺是脊椎动物中负责产生T细胞的主要淋巴器官，其适当的结构和组织对于最佳功能至关重要。我们的方法，多锥度圆平均光谱分析（MiCASA），识别组织水平组织的差异具有高灵敏度，包括通过测量变异性作为一个特定的参数定义一种新类型的表型。MiCASA为评估细胞组织提供了一种新颖且易于实施的定量工具。有几种方法可以量化组织图像中细胞类型之间的关系。在这里，作者提出了一种定量方法来评估细胞组织，在小鼠胸腺和脊髓中验证，称为多锥度圆平均光谱分析（MiCASA）。
While many tools exist for identifying and quantifying individual cell types, few methods are available to assess the relationships between cell types in organs and tissues and how these relationships change during aging or disease states. We present a quantitative method for evaluating cellular organization, using the mouse thymus as a test organ. The thymus is the primary lymphoid organ responsible for generating T cells in vertebrates, and its proper structure and organization is essential for optimal function. Our method, Multitaper Circularly Averaged Spectral Analysis (MiCASA), identifies differences in the tissue-level organization with high sensitivity, including defining a novel type of phenotype by measuring variability as a specific parameter. MiCASA provides a novel and easily implemented quantitative tool for assessing cellular organization. There are few methods available that can quantify relationships between cell types in tissue images. Here the authors present a quantitative method to evaluate cellular organization, validated in the mouse thymus and spinal cord, called Multitaper Circularly Averaged Spectral Analysis (MiCASA).