Characterisation of a near-infrared (NIR) fluorescence imaging systems intended for hybrid gamma-NIR fluorescence image guided surgery
Characterisation of a near-infrared (NIR) fluorescence imaging systems intended for hybrid gamma-NIR fluorescence image guided surgery
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DOI：
10.1088/1748-0221/14/07/p07007
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发表时间：
2019-07-01
影响因子：
1.3
通讯作者：
Perkins, A. C.
中科院分区：
文献类型：
--
作者：
Almarhaby, A. M.;Lees, J. E.;Perkins, A. C.
先前已经描述了一种新颖的手持式混合光学伽马相机（HGC），其能够在单个成像系统中显示来自两种模态的共同对准的图像。在这里，一个专用的近红外成像系统的近红外荧光手术指导已经开发出与HGC相结合。这项工作评估了两个近红外荧光成像系统使用幻影研究，各种荧光团和各种实验配置的性能。研究了ICG和800 CW染料在两种体系中的阈值检测浓度。构建模拟转移瘤的定制淋巴结模型和组织样层以评估检测能力。ICG可以检测到的最低浓度为1 μ M的每个相机。800 CW的较低阈值分别为10（-2）和10（-3）μ M的修改和NIR相机。这两种相机都无法检测到3 mm深度内的小尺寸目标，但能够识别深至7 mm的较大目标。需要进一步改进以优化NIR荧光系统，以便随后与HGC组合进行双伽马NIR荧光术中成像。
A novel hand-held hybrid optical-gamma camera (HGC) has previously been described that is capable of displaying co-aligned images from both modalities in a single imaging system. Here, a dedicated NIR imaging system for NIR fluorescence surgical guidance has been developed for combination with the HGC. This work has evaluated the performance of two NIR fluorescence imaging systems using phantom studies, various fluorophores and various experimental configurations. The threshold detectable concentration of ICG and 800CW dyes were investigated for both systems. Bespoke lymph node phantoms simulating metastases and tissue-like layers were constructed to evaluate the detection capability. ICG could be detected at a minimum concentration of 1 mu M for each camera. The lower thresholds for 800CW were 10(-2) and 10(-3) mu M for the modified and NIR cameras, respectively. Both cameras were unable to detect small-sized targets within a 3mm depth, but were able to identify larger targets as deep as 7 mm. Further improvements are required to optimise the NIR-fluorescence systems for subsequent combination with the HGC to undertake dual gamma-NIR fluorescence intraoperative imaging.