Transcriptomic Characterization, Chemosensitivity and Regulatory Effects of Exosomes in Spontaneous EMT/MET Transitions of Breast Cancer Cells
Transcriptomic Characterization, Chemosensitivity and Regulatory Effects of Exosomes in Spontaneous EMT/MET Transitions of Breast Cancer Cells
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DOI：
10.21873/cgp.20122
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发表时间：
2019-05-01
影响因子：
2.5
通讯作者：
Luceri, Cristina
中科院分区：
文献类型：
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作者：
Bigagli, Elisabetta;Cinci, Lorenzo;Luceri, Cristina
背景/目的：我们研究了乳腺癌细胞自发进行上皮-间质转化（EMT）及其逆向过程间质-上皮转化（MET）的基因表达变化以及外泌体在这些转变中的作用。材料和方法：采用微阵列基因表达谱、免疫细胞化学和化学敏感性对高侵袭性间充质样乳腺癌细胞MDA-MB-231（基底细胞）、EMT和MET变异进行表征。结果：自发播散的细胞具有抗风疹性，表现出解离性，emt样表型，并在无细胞板中重新播种时发生MET。基底细胞分泌外泌体抑制MET。对阿霉素、长春新碱和紫杉醇的化疗敏感性随EMT的顺序而降低
Background/Aim: We examined the gene expression changes of breast cancer cells spontaneously undergoing epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) and the role of exosomes in these transitions. Materials and Methods: Highly invasive mesenchymal-like breast cancer cells, MDA-MB-231 (basal cells), EMT and MET variants, were characterized by microarray gene expression profiling, immuno cytochemistry and chemo-sensitivity. Results: Spontaneously disseminated cells were anoikis resistant, exhibited a dissociative, EMT-like phenotype and underwent MET when reseeded in cell-free plates. MET was inhibited by exosomes secreted by basal cells. Chemo-sensitivity to doxorubicin, vincristine and paclitaxel decreased in the order EMT