Distribution and concentration of TRH in the rat brain.
Distribution and concentration of TRH in the rat brain.
复制标题
TRH 在大鼠脑中的分布和浓度。
DOI：
10.1210/endo-95-2-540
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发表时间：
1974
期刊：
影响因子：
4.8
通讯作者：
J. C. Porter
中科院分区：
文献类型：
--
作者：
C. Oliver;R. Eskay;N. Ben;J. C. Porter
定性和定量标准表明，TRH是目前从所有地区的大鼠脑制备的甲醇提取物。大鼠脑提取物中的TRH与合成TRH相同的结论基于以下标准：（a）与合成TRH的特异性抗血清的免疫反应性，（B）在Sephadex G-10上的层析特性，（c）在醋酸纤维素上的电泳迁移率，（d）对血浆降解的敏感性，和（e）提取物在体外刺激垂体前叶组织释放TSH的能力。用放射免疫分析法测定TRH的含量，发现下丘脑最高，其次是垂体后叶、丘脑、脑干、大脑、垂体前叶和小脑。大鼠脑内TRH总量为18.2ng。其中丘脑占33.6%，大脑占26.7%，下丘脑占18.8%，脑干占18.6%，小脑占1.6%，垂体后叶占0.42%。
Qualitative and quantitative criteria indicate that TRH is present in methanolic extracts prepared from all regions of the rat brain. The conclusion that TRH in the extracts of rat brain is the same as synthetic TRH is based on the following criteria: (a) immunoreactivity with a specific antiserum to synthetic TRH, (b) chromatographic properties on Sephadex G-10, (c) electrophoretic mobility on cellulose acetate, (d) susceptibility to degradation by plasma, and (e) capacity of the extracts to stimulate TSH release in vitro from anterior pituitary tissue. The greatest concentration of TRH, determined by radioirnmunoassay, was found in the hypothalamus, followed by the posterior pituitary gland, thalamus, brain stem, cerebrum, anterior pituitary gland, and cerebellum. The total quantity of TRH in the rat brain was 18.2 ng. Of this amount, 33.6% was present in the thalamus; 26.7% in the cerebrum; 18.8% in the hypothalamus; 18.6% in the brain stem; 1.6% in the cerebellum; 0.42% in the posterior pituitary glan...