Cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as BamHI fragments.
Cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as BamHI fragments.
复制标题
将重复和非重复单纯疱疹病毒 1 序列克隆为 BamHI 片段。
DOI：
10.1073/pnas.77.7.4201
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发表时间：
1980
影响因子：
11.1
通讯作者：
Roizman,B
中科院分区：
文献类型：
--
作者：
Post,LE;Conley,AJ;Mocarski,ES;Roizman,B
在pBR322中，95%以上的单纯疱疹病毒1型(F株)DNA序列被克隆为BamHI片段。除了一个例外，所有克隆的片段与来源于病毒基因组BamHI酶切的正品片段具有相同的电泳迁移率和限制性内切酶切割位点。例外的是在DNA的原型排列中L成分右端的BamHI B片段映射。因此，在两个独立衍生的质粒中，片段左端附近存在一个小的缺失图谱。该质粒集合中包括几个克隆，它们包含跨越病毒DNA的L和S组成部分之间的连接的dna序列。含有连接片段的几个质粒被发现足够稳定，以允许制备大量的DNA片段，用于限制酶切割位点的精细结构图谱。对一个克隆片段(BamHI G)的初步研究表明，它在标记挽救温度敏感突变和转移斑块形态标记方面具有生物活性。
Over 95% of the herpes simplex virus type 1 (strain F) DNA sequences have been cloned as BamHI fragments in the pBR322 plasmid. With one exception, all of the cloned fragments have the same electrophoretic mobilities and restriction enzyme cleavage sites as do the authentic fragments derived from the BamHI digests of the viral genome. The exception is the BamHI B fragment mapping at the right end of L component in the prototype arrangement of the DNA. Thus, a small deletion mapping near the left end of the fragment was present in two independently derived plasmids. Included in the collection of plasmids are several clones containing DNA sequences that span the junction between the L and S components of the virus DNA. Several plasmids containing the junction fragment were found to be sufficiently stable to permit the preparation of large amounts of the DNA fragment for fine-structure mapping of the restriction enzyme cleavage sites. Preliminary studies on one cloned fragment (BamHI G) have shown that it is biologically active in marker rescue of a temperature-sensitive mutation and in transfer of a plaque morphology marker.