On-Demand Electrochemical Activation of the Click Reaction on Self-Assembled Monolayers on Gold Presenting Masked Acetylene Groups
On-Demand Electrochemical Activation of the Click Reaction on Self-Assembled Monolayers on Gold Presenting Masked Acetylene Groups
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DOI：
10.1021/ja2083587
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发表时间：
2011-10-26
影响因子：
15
通讯作者：
Yeo, Woon-Seok
中科院分区：
文献类型：
--
作者：
Choi, Inseong;Kim, Young-Kwan;Yeo, Woon-Seok
我们报告了一种新的表面改性方法，用于嫁接“动态”特性，从而按需激活点击反应。我们的方法利用六羰基二钴 Co-2-(CO)(6) 掩蔽的乙炔基团，该基团对点击反应不发生反应。电化学处理揭示了所选区域上的乙炔基团，然后通过与含叠氮分子的点击反应将其用作表面功能化的化学手柄。通过循环伏安法、X射线光电子能谱和荧光光谱验证了表面的电化学和化学转化。我们已经证明了叠氮化物修饰的 RGD 肽的固定化和促进细胞粘附/迁移到电化学诱导区域的作用。
We report on a new surface modification method for grafting a "dynamic" property for on-demand activation of the click reaction. Our approach utilizes the acetylene group masked with dicobalt hexacarbonyl, Co-2-(CO)(6), which is not reactive toward the click reaction. Electrochemical treatment reveals the acetylene group on the selected region, which is then used as a chemical handle for surface functionalization via the click reaction with an azide-containing molecule. Electrochemical and chemical conversions on the surface were verified by cyclic voltammetry, X-ray photoelectron spectroscopy, and fluorescence spectroscopy. We have demonstrated immobilization of an azide-modified RGD peptide and promotion of cell adhesion/migration to the region of electrochemical induction.