New Insights into BAR Domain-Induced Membrane Remodeling
New Insights into BAR Domain-Induced Membrane Remodeling
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DOI：
10.1016/j.bpj.2009.06.036
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发表时间：
2009-09-16
影响因子：
3.4
通讯作者：
Voth, Gregory A.
中科院分区：
文献类型：
--
作者：
Ayton, Gary S.;Lyman, Edward;Voth, Gregory A.
利用介观模拟和电子显微镜对N-bar结构域诱导的脂质体重塑过程进行了研究。膜重塑的整个过程涉及N-bar蛋白密度、N-bar寡聚化程度和膜密度之间的复杂耦合。介观模拟结果与电子显微镜实验结果的比较表明，膜的重塑过程可以通过合适的介观自由能框架来描述。在介观模拟框架内，还提出了更具代表性的F-bar结构域的脂质体重塑。
Mesoscopic simulations and electron microscopy of N-BAR domain-induced liposome remodeling are used to characterize the process of liposome tubulation and vesiculation. The overall process of membrane remodeling is found to involve complex couplings among the N-BAR protein density, the degree of N-BAR oligomerization, and the membrane density. A comparison of complex remodeled liposome structures from mesoscopic simulations with those measured by electron microscopy experiments suggests that the process of membrane remodeling can be described via an appropriate mesoscopic free energy framework. Liposome remodeling more representative of F-BAR domains is also presented within the mesoscopic simulation framework.