ACTIVATION BY POLYAMINES OF THE ACETYLATION OF ENDOGENOUS HISTONES IN ISOLATED CHROMATIN AND NUCLEI FROM ARTEMIA
ACTIVATION BY POLYAMINES OF THE ACETYLATION OF ENDOGENOUS HISTONES IN ISOLATED CHROMATIN AND NUCLEI FROM ARTEMIA
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DOI：
10.1111/j.1432-1033.1981.tb05626.x
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
PESTANA, A
中科院分区：
文献类型：
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作者：
ESTEPA, I;PESTANA, A
多胺的乙酰基转移率从乙酰辅酶A内源性组蛋白的染色质和细胞核从50小时龄卤虫无节幼体，与脱乙酰化率的变化不大。在这些测定条件下，内源性乙酰转移酶没有乙酰化多胺。多胺的激活表现出双重特异性的效应，精胺，和基板，组蛋白H3。当在亚饱和底物浓度下测定时，发现分离的组蛋白乙酰转移酶的激活具有相同的特异性。发现半最大活化所需的精胺浓度为50-60 μ M。
Polyamines increased the rate of transfer of acetyl groups from acetyl-CoA to endogenous histones in chromatin and nuclei from 50 h old Artemia nauplii, with little changes in the rate of deacetylation. Polyamines were not acetylated by endogenous acetyltransferases in these assay conditions. The activation by polyamines showed a double specificity for both the effector, spermine, and the substrate, the histone H3. The same specificity was found for the activation of isolated histone acetyltransferase when assayed at subsaturating substrate concentration. The concentration of spermine required for half-maximal activation was found to be 50-60 .mu.M.