Development of endothelial cell lines from embryonic stem cells - A tool for studying genetically manipulated endothelial cells in vitro
Development of endothelial cell lines from embryonic stem cells - A tool for studying genetically manipulated endothelial cells in vitro
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DOI：
10.1161/01.atv.20.6.1443
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发表时间：
2000-06-01
影响因子：
8.7
通讯作者：
Dejana, E
中科院分区：
文献类型：
--
作者：
Balconi, G;Spagnuolo, R;Dejana, E
全能胚胎干细胞在体外可被诱导分化为内皮细胞。这可能是获得基因操纵内皮细胞培养的有用工具，因为胚胎干细胞相对容易转化，通常用于小鼠的基因失活实验，但胚胎干细胞来源的内皮细胞不容易从类胚体中分离出来并在培养中保持。在这项研究中，我们描述了从体外分化的胚胎干细胞获得的永生化内皮细胞系的分离酸特性。分析细胞系内皮细胞标志物的表达，包括生长因子受体和黏附分子，并与从卵黄囊、胚胎本身或成人心脏微循环中获得的内皮细胞进行比较。我们认为，这种方法可能有助于获得携带基因突变的内皮细胞，这些突变在发育的早期阶段是致命的。
Totipolent embryonic stem cells can be induced to differentiate to endothelium in vitro. This may be a useful tool for obtaining cultures of genetically manipulated endothelial cells because embryonic stem cells are relatively easy to transfect and are commonly used for gene inactivation experiments in mice, However, embryonic stem cell-derived endothelial cells could not be easily separated from embryoid bodies and maintained in culture. In this study, we describe the isolation acid characterization of immortalized endothelial cell lines obtained from embryonic stem cells differentiated in vitro. The cell lines were analyzed for expression of endothelial cell markers, including growth factor receptors and adhesion molecules, and compared with endothelial cells obtained from the yolk sac, the embryo proper, or the heart microcirculation of the adult. We propose that this approach may be useful for obtaining endothelial cells carrying gene mutations that are lethal at very early stages of development.