Chicken cathelicidin-B1, an antimicrobial guardian at the mucosal M cell gateway
Chicken cathelicidin-B1, an antimicrobial guardian at the mucosal M cell gateway
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DOI：
10.1073/pnas.0707037104
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发表时间：
2007-09-18
影响因子：
11.1
通讯作者：
Cooper, Max D.
中科院分区：
文献类型：
--
作者：
Goitsuka, Ryo;Chen, Chen-Io H.;Cooper, Max D.
粘液上皮M细胞为微生物提供了一个有效的入口。最初由它们的不规则微绒毛和丰富的胞吞通道在禽法氏囊中定义，M细胞也被发现在哺乳动物阑尾的淋巴滤泡相关上皮、派伊尔集合淋巴结和其他粘膜表面淋巴界面中。我们在这里描述了一个以前未被识别的cathelicidin基因在鸡，chCATH-B1，这是专门表达在上皮细胞的法氏囊。与先前鉴定的凯萨林菌素的成熟肽一样，chCATH-B1的羧基末端肽对革兰氏阳性和革兰氏阴性细菌具有广泛的抗微生物活性。chCATH-B1表达仅限于M细胞的分泌上皮细胞邻居，而其成熟肽被转运集中在覆盖囊淋巴滤泡的M细胞基底外侧表面周围的纤维状网络上。我们的结论是，chCATH-B1是很好的位置，在M细胞网关提供保护性抗菌作用。
Mucosal epithelial M cells provide an efficient portal of entry for microorganisms. Initially defined by their irregular microvilli and abundant transcytotic channels in the avian bursa of Fabricius, M cells also are found in the lymphoid follicle-associated epithelium of the mammalian appendix, Peyer's patches, and other mucosal surface-lymphoid interfaces. We describe here a previously unrecognized cathelicidin gene in chickens, chCATH-B1, that is expressed exclusively in the epithelium of the bursa of Fabricius. Like the mature peptides of previously identified cathelicidins, the carboxyl-terminal peptide of chCATH-B1 has broad antimicrobial activity against Gram-positive and Gram-negative bacteria. chCATH-B1 expression is restricted to the secretory epithelial cell neighbors of the M cells, whereas its mature peptide is transported to become concentrated on the fibrillar network surrounding basolateral surfaces of the M cells that overlie the bursal lymphoid follicles. We conclude that chCATH-B1 is well placed to serve a protective antimicrobial role at the M cell gateway.