Elevated levels of miR-146a and miR-155 in kidney biopsy and urine from patients with IgA nephropathy
Elevated levels of miR-146a and miR-155 in kidney biopsy and urine from patients with IgA nephropathy
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IgA 肾病患者肾活检和尿液中 miR-146a 和 miR-155 水平升高
DOI：
10.1155/2011/304852
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Szeto, Cheuk-Chun
中科院分区：
文献类型：
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作者：
Wang, Gang;Kwan, Bonnie Ching-Ha;Szeto, Cheuk-Chun
背景：已有研究表明miR-146a和miR-155在先天免疫和适应性免疫应答中发挥重要作用。我们研究了免疫球蛋白A肾病（IgAN）患者肾内和尿内miR-146a和miR-155的水平。方法：量化43例IgAN患者肾内和尿内miR-146a和miR-155水平；结果与20个肾切除标本和13名健康志愿者的尿液沉积物进行了比较。结果：IgAN肾内和尿内miR-146a和miR-155水平显著高于对照组。肾小球滤过率与肾内miR-146a和miR-155水平呈负相关；蛋白尿与肾内miR-146a和miR-155水平呈正相关。以及尿中miR-146a和miR-155的水平。肾内miR-155水平与小管间质瘢痕形成显著相关。尿中miR-146a水平与尿中白细胞介素(IL)-1 β、IL-6和肿瘤坏死因子(TNF)- α的表达呈负相关，与尿中活化后调节t细胞表达、正常t细胞表达和分泌（RANTES）呈正相关。尿中miR-155水平与尿中IL-1 β和tnf - α的表达呈负相关，与尿中叉头盒P3 （FOXP3）和RANTES的表达呈正相关。结论：我们得出结论，在IgAN中，肾内和尿内miR-146a和miR-155水平显著升高，且上调程度与疾病的临床和组织学严重程度相关。我们的结果提示miR-146a和miR-155可能在IgAN的病理生理中发挥重要作用。
Background: Previous studies suggested miR-146a and miR-155 play important roles in innate and adaptive immune responses. We studied intra-renal and urinary levels of miR-146a and miR-155 in patients with immunoglobulin A nephropathy (IgAN).Methods: Intra-renal and urinary levels of miR-146a and miR-155 are quantified in 43 patients with IgAN; the result was compared to 20 nephrectomy specimens and urine sediment of 13 healthy volunteers.Results: The levels of intra-renal and urinary levels of miR-146a and miR-155 of IgAN are significantly higher than controls. Estimated glomerular filtration rate inversely correlates with intra-renal level of miR-146a and miR-155; proteinuria positively correlates with intra-renal level of miR-146a and miR-155. as well as urinary level of miR-146a and miR-155. Intra-renal level of miR-155 significantly correlates with tubulointerstitial scarring. Urinary level of miR-146a inversely correlates with urinary expression of interleukin (IL)-1 beta, IL-6 and tumor necrosis factor (TNF)-alpha and positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES). Urinary level of miR-155 inversely correlates with urinary expression of IL-1 beta and TNF-alpha and positively correlates with urinary expression of forkhead box P3 (FOXP3) and RANTES.Conclusion: We conclude that intra-renal and urinary levels of miR-146a and miR-155 were significantly elevated in IgAN, and the degree of upregulation correlates with clinical and histological severity of the disease. Our results suggested miR-146a and miR-155 might play an important role in the pathophysiology of IgAN.