THE INACTIVE X-CHROMOSOME IN FEMALE MAMMALS IS DISTINGUISHED BY A LACK OF HISTONE-H4 ACETYLATION, A CYTOGENETIC MARKER FOR GENE-EXPRESSION
THE INACTIVE X-CHROMOSOME IN FEMALE MAMMALS IS DISTINGUISHED BY A LACK OF HISTONE-H4 ACETYLATION, A CYTOGENETIC MARKER FOR GENE-EXPRESSION
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DOI：
10.1016/0092-8674(93)90419-q
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发表时间：
1993-07-30
期刊：
影响因子：
64.5
通讯作者：
TURNER, BM
中科院分区：
文献类型：
--
作者：
JEPPESEN, P;TURNER, BM
我们用组蛋白H4的乙酰化亚型特异性抗体免疫标记了人类和小鼠的中期染色体。除了雌性细胞中的一条染色体外，所有染色体都在与常规R带（富含编码DNA的区域）相对应的区域进行了标记，该染色体大部分未被标记，我们将其鉴定为非活性X（Xi）。三个明确定义的免疫荧光带，增强丁酸预处理，观察到在同源位置上的人类和小鼠的Xi，显示有限的，区域持久性的H4乙酰化。其中两条带位于已知含有Xi表达基因的细胞遗传学区域。我们建议，H4超乙酰化定义的基因组区域含有潜在的转录活性染色质，而虚拟的H4乙酰化定义组成性和兼性异染色质。
We have immunolabeled human and mouse metaphase chromosomes with antibodies specific for the acetylated isoforms of histone H4. All chromosomes were labeled in regions corresponding to conventional R bands (regions enriched in coding DNA), except for a single chromosome in female cells, which was largely unlabeled and which we have identified as the inactive X (Xi). Three sharply defined immunofluorescent bands, enhanced by butyrate pretreatment, were observed in homologous positions on the human and mouse Xi, showing limited, regional persistence of H4 acetylation. Two of these bands are in cytogenetic regions known to contain genes expressed on Xi. We propose that H4 hyperacetylation defines regions of the genome containing potentially transcriptionally active chromatin, while virtual absence of H4 acetylation defines both constitutive and facultative heterochromatin.