Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory
Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory
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DOI：
10.1046/j.1365-313x.1998.00308.x
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发表时间：
1998-11-01
期刊：
影响因子：
7.2
通讯作者：
Lida, S
中科院分区：
文献类型：
--
作者：
Habu, Y;Hisatomi, Y;Lida, S
常见的牵牛花易变的片状（或a(f)）线长有白色的花，有彩色的薄片和扇形。a(f)等位基因显示不完全显性。杂合状态下的植株A/ A (f)有浅色的花，带有强烈颜色的薄片，偶尔有白色的扇区。我们发现可变的a(f)等位基因是由一个新的转座元件Tip100插入到CHS-D基因内含子中引起的。Tip100长3.9 kb，属于Ac/Ds系列。虽然花的时间和频率在不同的品系中有所不同，但它们携带相同的可变等位基因。我们还注意到，一个带有杂色花的片状亚系携带Tip100的衍生物Tip100-1， Tip100-1的结构包含一个额外的48bp的末端序列作为串联重复序列，其整合位点与Tip100相同。另一个有着稳定白花的品系是双突变体，携带两个拷贝的CHS-D基因Tip100。这些结果是讨论关于花的杂色表型在各种可变系。
The mutable flaked (or a(f)) lines of the common morning glory bear white flowers with colored flakes and sectors. The a(f) allele shows incomplete dominance. Plants in the heterozygous state A/a(f) bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable a(f) allele is caused by insertion of a new transposable element, Tip100, into the CHS-D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100-1 The structure of Tip100-1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100. Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS-D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.