Planarian d-amino acid oxidase is involved in ovarian development during sexual induction
Planarian d-amino acid oxidase is involved in ovarian development during sexual induction
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DOI：
10.1016/j.mod.2013.12.003
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发表时间：
2014-05
影响因子：
2.6
通讯作者：
T. Maezawa;Hiroyuki Tanaka;Haruka Nakagawa;Mizuki Ono;Manabu Aoki;M. Matsumoto;T. Ishida;K. Horiike;K. Kobayashi
中科院分区：
文献类型：
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作者：
T. Maezawa;Hiroyuki Tanaka;Haruka Nakagawa;Mizuki Ono;Manabu Aoki;M. Matsumoto;T. Ishida;K. Horiike;K. Kobayashi
为了阐明无性生殖向有性生殖转变的分子机制，即性诱导，我们建立了一个两性真涡虫种琉球三角涡虫（Dugesia ryukyuensis）性诱导的检测系统。卵巢发育是性诱导的最初和重要步骤，随后是其他生殖器官的形成，包括睾丸。在这里，我们报告了一个功能的Planariand-amino酸氧化酶，博士DAO，在控制卵巢发育的Planarians。无性蠕虫表现出更广泛的表达的Dr-DAO的实质空间比有性蠕虫。通过RNAi抑制Dr-DAO导致未成熟卵巢的形成。此外，我们发现，喂养无性繁殖的蠕虫5种特定的氨基酸可以诱导未成熟卵巢的形成，这与在Dr-DAO敲除蠕虫中观察到的相似，这表明Dr-DAO通过降解这些氨基酸来抑制未成熟卵巢的形成。在性诱导后，在卵巢中观察到Dr-DAO表达。在性诱导过程中，Dr-DAO的敲低延迟了其他生殖器官以及卵巢的成熟。这些研究结果表明，Dr-DAO促进卵巢成熟，完全的性诱导取决于成熟卵巢的产生。我们认为，体细胞中产生的Dr-DAO阻止了无性状态下性诱导的发生，然后在性诱导后，雌性生殖细胞特异性地产生Dr-DAO以诱导完全成熟。因此，体细胞和雌性生殖细胞中产生的Dr-DAO可能在性诱导中发挥不同的作用。
To elucidate the molecular mechanisms underlying switching from asexual to sexual reproduction, namely sexual induction, we developed an assay system for sexual induction in the hermaphroditic planarian speciesDugesia ryukyuensis. Ovarian development is the initial and essential step in sexual induction, and it is followed by the formation of other reproductive organs, including the testes. Here, we report a function of a planariand-amino acid oxidase, Dr-DAO, in the control of ovarian development in planarians. Asexual worms showed significantly more widespread expression ofDr-DAOin the parenchymal space than did sexual worms. Inhibition ofDr-DAOby RNAi caused the formation of immature ovaries. In addition, we found that feeding asexual worms 5 specificd-amino acids could induce the formation of immature ovaries that are similar to those observed inDr-DAOknockdown worms, suggesting that Dr-DAO inhibits the formation of immature ovaries by degrading thesed-amino acids. Following sexual induction,Dr-DAOexpression was observed in the ovaries. The knockdown ofDr-DAOduring sexual induction delayed the maturation of the other reproductive organs, as well as ovary. These findings suggest that Dr-DAO acts to promote ovarian maturation and that complete sexual induction depends on the production of mature ovaries. We propose that Dr-DAO produced in somatic cells prevents the onset of sexual induction in the asexual state, and then after sexual induction, the female germ cells specifically produce Dr-DAO to induce full maturation. Therefore, Dr-DAO produced in somatic and female germline cells may play different roles in sexual induction.