Effect of sialyllactose administration on growth performance and intestinal epithelium development in suckling piglets
Effect of sialyllactose administration on growth performance and intestinal epithelium development in suckling piglets
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DOI：
10.1016/j.anifeedsci.2022.115205
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发表时间：
2022-01-10
影响因子：
3.2
通讯作者：
He, Jun
中科院分区：
文献类型：
--
作者：
Li, Dengfu;Chen, Daiwen;He, Jun
唾液酸乳糖 (SL) 是母猪初乳中丰富的碳水化合物，但在哺乳期的最初几天会减少。在这里，我们研究了它对哺乳期猪的生长性能和肠上皮发育的影响。将 16 窝哺乳仔猪分为两组（对照组和 SL 组）。所有仔猪均采用母乳喂养，SL组每天口服4 g SL，连续21 d。结果显示，SL 施用增加了窝重（P = 0.043），并且倾向于提高断奶时的平均体重（P = 0.084）。 SL给药后血清免疫球蛋白G和谷胱甘肽过氧化物酶浓度升高，但肿瘤坏死因子α、丙二醛和二胺氧化酶浓度降低（P < 0.05）。此外，SL给药提高了绒毛高度、绒毛高度与隐窝深度的比值以及空肠中乳糖酶和蔗糖酶的活性（P < 0.05），并减少了回肠上皮中的凋亡细胞总数（P = 0.003）。补充SL可提高空肠和回肠中紧密连接蛋白ZO-1的表达水平，以及空肠中葡萄糖转运蛋白2、二价金属转运蛋白1和胰岛素生长因子1受体等关键功能基因的表达水平（P < 0.05）。重要的是，SL 增加了丁酸浓度，并提高了盲肠中乳酸杆菌和双歧杆菌的丰度（P < 0.05）。这些结果表明，SL 可以改善哺乳仔猪的生长性能，这与提高免疫力和抗氧化能力以及肠道屏障功能有关。
Sialyllactose (SL) is an abundant carbohydrate in sow's colostrum, but decreases during the first few days of lactation. Here, we investigated its effect on growth performance and intestinal epithelium development in pigs during the suckling period. Sixteen litters of suckling piglets were divided into two groups (Control and SL group). All piglets were breast fed and the SL group was orally administrated with 4 g SL per day for 21 d. Results showed that SL administration increased the litter weight (P = 0.043) and tended to elevate the average body weight (P = 0.084) at weaning. The serum concentrations of immunoglobulin G and glutathione peroxidase were elevated, but the concentrations of tumor necrosis factor alpha, malondialdehyde, and diamine oxidase were decreased upon SL administration (P < 0.05). Moreover, SL administration elevated the villus height, the ratio of villus height to crypt depth, and the activities of lactase and sucrase in jejunum (P < 0.05), and decreased the total apoptotic cells in ileal epithelium (P = 0.003). The expression levels of tight-junction protein ZO-1 in the jejunum and ileum, and critical functional genes such as the glucose transporter 2, divalent metal transporter 1, and insulin growth factor 1 receptor in the jejunum were elevated by SL supplementation (P < 0.05). Importantly, SL increased butyric acid concentration, and elevated the abundances of Lactobacillus and Bifidobacterium in cecum (P < 0.05). These results suggest that SL could improve the growth performance of suckling piglets, which was associated with improved immunity and antioxidant capacity, as well as the intestinal barrier functions.