Correlation between nucleotide mutation and viral loads of human bocavirus 1 in hospitalized children with respiratory tract infection
Correlation between nucleotide mutation and viral loads of human bocavirus 1 in hospitalized children with respiratory tract infection
复制标题
呼吸道感染住院儿童人博卡病毒1型核苷酸突变与病毒载量的相关性
DOI：
10.1099/vir.0.047472-0
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发表时间：
2013-05-01
影响因子：
3.8
通讯作者：
Liu, Enmei
中科院分区：
文献类型：
--
作者：
Hao, Rui;Ni, Ke;Liu, Enmei
人类博卡病毒1（HBoV 1）细小病毒引起呼吸道疾病，主要影响儿童。尽管其在世界范围内流行，但HBoV 1复制和发病机制在很大程度上仍不明确。在这项研究中，2009年6月至2011年5月期间在中国重庆医科大学儿童医院因呼吸道感染住院的846名儿童中，通过实时定量PCR检测到112名（13.2%）HBoV 1。HBoV 1阳性患者的中位年龄为10个月。45例（40.2%）HBoV 1病例为单一感染，67例（59.8%）为病毒合并感染。对112例HBoV 1阳性病例进行基因分型，获得27个HBoV 1全序列，以及2个NS 1基因序列、15个NP 1基因序列和10个VP 1/VP 2基因序列。分别为24、10和43个突变。统计学分析显示基因突变与HBoV 1阳性患者的临床表现无关。然而，在NP 1中具有突变G236 A或A447 G或在VP 1/VP 2中具有突变G1461 A的样品中的病毒载量显著低于具有野生型HBoV 1的样品。未来的研究应该调查HBoV 1基因中的这些突变是否可能代表疾病发病机制的有用标志物。
The human bocavirus 1 (HBoV1) parvovirus causes respiratory disease and primarily affects children. Despite its worldwide prevalence, the mechanisms of HBoV1 replication and pathogenesis remain largely undefined. In this study of 846 children hospitalized at the Children's Hospital of Chongqing Medical University in China for respiratory tract infection between June 2009 and May 2011, HBoV1 was detected in 112 (13.2%) by real-time quantitative PCR. The median age of HBoV1-positive patients was 10 months old. Forty-five (40.2%) of the HBoV1 cases were monoinfections, and 67 (59.8%) were viral co-infections. Genotyping of all 112 HBoV1-positive cases yielded 27 full HBoV1 sequences, as well as two NS1 gene sequences, 15 NP1 gene sequences and 10 VP1/VP2 gene sequences harbouring. 24, 10 and 43 mutations, respectively. Statistical analysis revealed no relationship between genetic mutations and clinical manifestations of HBoV1-positive patients. However, the viral loads were significantly lower in samples with mutations G236A or A447G in NP1, or G1461A in VP1/VP2, than in samples with wild-type HBoV1. Future studies should investigate whether these mutations in the HBoV1 gene may represent useful markers of disease pathogenesis.