Nucleotide sequencing and serological evidence that the recently recognized deer tick virus is a genotype of Powassan virus
Nucleotide sequencing and serological evidence that the recently recognized deer tick virus is a genotype of Powassan virus
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DOI：
10.1016/s0168-1702(01)00330-6
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发表时间：
2001-11-05
期刊：
影响因子：
5
通讯作者：
Barrett, ADT
中科院分区：
文献类型：
--
作者：
Beasley, DWC;Suderman, MT;Barrett, ADT
鹿蜱病毒（DTV）是最近从北美硬蜱中分离出来的一种病毒。对结构和非结构蛋白基因片段的核苷酸测序表明，这种病毒与蜱传黄病毒 Powassan (POW) 关系最密切，后者可能导致人类致命的脑炎。为了确定 DTV 是否代表黄病毒科黄病毒属的新的独特成员，我们对该病毒的结构蛋白基因以及 5' 和 3' 非编码区进行了测序。此外，我们还比较了DTV和POW在血凝抑制试验中与一组多克隆和单克隆抗血清的反应性，并使用抗DTV抗血清进行了交叉中和实验。核苷酸测序显示DTV和POW在核苷酸（> 80％同源性）和氨基酸（> 90％同源性）水平上具有高度同源性，并且两种病毒在血清学检测和小鼠神经侵袭性中无法区分。根据这些结果，我们建议将 DTV 归类为 POW 病毒的一个基因型。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Deer tick virus (DTV) is a recently recognized North American virus isolated from Ixodes dammini ticks. Nucleotide sequencing of fragments of structural and non-structural protein genes suggested that this virus was most closely related to the tick-borne flavivirus Powassan (POW), which causes potentially fatal encephalitis in humans. To determine whether DTV represents a new and distinct member of the Flavivirus genus of the family Flaviviridae, we sequenced the structural protein genes and 5 ' and 3 ' non-coding regions of this virus. In addition, we compared the reactivity of DTV and POW in hemagglutination inhibition tests with a panel of polyclonal and monoclonal antisera, and performed cross-neutralization experiments using anti-DTV antisera. Nucleotide sequencing revealed a high degree of homology between DTV and POW at both nucleotide (> 80% homology) and amino acid (> 90% homology) levels, and the two viruses were indistinguishable in serological assays and mouse neuroinvasiveness. On the basis of these results, we suggest that DTV should be classified as a genotype of POW virus. (C) 2001 Elsevier Science B.V. All rights reserved.