Discovery of Insect Attractants Based on the Functional Analyses of Female-Biased Odorant Receptors and Their Orthologs in Two Closely Related Species
Discovery of Insect Attractants Based on the Functional Analyses of Female-Biased Odorant Receptors and Their Orthologs in Two Closely Related Species
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DOI：
10.1021/acs.jafc.3c05368
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发表时间：
2023-12-01
影响因子：
6.1
通讯作者：
Wang，Chen-Zhu
中科院分区：
文献类型：
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作者：
Mo，Bao-Tong;Guo，Hao;Wang，Chen-Zhu
嗅觉在植食性昆虫选择寄主植物中起着重要作用，烟夜蛾和棉铃虫是寄主范围不同、亲缘关系较近的两种蛾。在本研究中，我们首先利用果蝇T1神经元表达系统比较分析了两个物种中11个雌性偏向气味受体(ORs)及其同源基因的功能，然后检测了这两个物种对最有效的OR配体的触角电位反应。用Y管嗅觉仪进行的行为分析表明，HassOR21-2和HarmOR21-2的主要配体愈创木酚只吸引雌性，而HassOR35和HarmOR35的主要配体苄基丙酮吸引两种雌性。产卵选择性实验进一步证实愈创木烯和苄基丙酮是两种交配雌虫的有效产卵引诱剂。这些发现加深了我们对食草性昆虫寄主植物选择的嗅觉编码机制的理解，并为管理害虫种群提供了有价值的引诱剂。
Olfaction plays an instrumental role in host plant selection by phytophagous insects.Helicoverpa assultaandHelicoverpa armigeraare two closely related moth species with different host plant ranges. In this study, we first comparatively analyzed the function of 11 female-biased odorant receptors (ORs) and their orthologs in the two species by theDrosophilaT1 neuron expression system and then examined the electroantennography responses of the two species to the most effective OR ligands. Behavioral assays using a Y-tube olfactometer indicate that guaiene, the primary ligand of HassOR21–2 and HarmOR21–2, only attracts the females, while benzyl acetone, the main ligand of HassOR35 and HarmOR35, attracts both sexes of the two species. Oviposition preference experiments further confirm that guaiene and benzyl acetone are potent oviposition attractants for the mated females of both species. These findings deepen our understanding of the olfactory coding mechanisms of host plant selection in herbivorous insects and provide valuable attractants for managing pest populations.