Partial resistance to gD-mediated interference conferred by mutations affecting herpes simplex virus type 1 gC and gK.
Partial resistance to gD-mediated interference conferred by mutations affecting herpes simplex virus type 1 gC and gK.
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对 gD 介导的干扰具有部分抵抗力，该干扰是由影响单纯疱疹病毒 1 型 gC 和 gK 的突变赋予的。
DOI：
10.1128/jvi.71.10.8024-8028.1997
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发表时间：
1997
影响因子：
5.4
通讯作者：
Spear,PG
中科院分区：
文献类型：
--
作者：
Pertel,PE;Spear,PG
表达单纯疱疹病毒（HSV）gD的细胞可由于gD介导的干扰而对HSV进入具有抗性。HSV毒株对这种干扰的敏感性不同，这种干扰阻断病毒渗透但不结合。先前的研究表明，病毒体相关gD的突变或变异可赋予对gD介导的干扰的抗性。在这里，我们表明，HSV-1的突变体选择增强的能力，结合和渗透在抑制浓度的肝素的存在下，部分抵抗gD介导的干扰。耐药性主要是由于存在两种突变：一种是gC（主要的肝素结合糖蛋白）突变，导致gC表达缺失，另一种是gK突变，导致合胞体表型。结果表明，肝素选择的突变体与改变后结合的要求进入。先前尚未描述由影响gC和gK的突变赋予的对gD介导的干扰的抗性。
Cells expressing herpes simplex virus (HSV) gD can be resistant to HSV entry as a result of gD-mediated interference. HSV strains differ in sensitivity to this interference, which blocks viral penetration but not binding. Previous studies have shown that mutations or variations in virion-associated gD can confer resistance to gD-mediated interference. Here we show that HSV-1 mutants selected for enhanced ability to bind and penetrate in the presence of inhibitory concentrations of heparin were partially resistant to gD-mediated interference. The resistance was largely due to the presence of two mutations: one in gC (the major heparin-binding glycoprotein) resulting in the absence of gC expression and the other in gK resulting in a syncytial phenotype. The results imply that heparin selected for mutants with altered postbinding requirements for entry. Resistance to gD-mediated interference conferred by mutations affecting gC and gK has not been previously described.