DNA damage and plasma antioxidant indices in Bangladeshi type 2 diabetic patients
DNA damage and plasma antioxidant indices in Bangladeshi type 2 diabetic patients
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DOI：
10.1016/j.diabet.2009.05.007
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发表时间：
2010-02-01
影响因子：
7.2
通讯作者：
Kabir, Y.
中科院分区：
文献类型：
--
作者：
Arif, M.;Islam, M. R.;Kabir, Y.
背景和目标。糖尿病是一种复杂的代谢性疾病，其特征是糖代谢紊乱，最近的证据表明，氧化应激增加以及氧化能力的改变可能与2型糖尿病患者的并发症有关。本研究的目的是检测2型糖尿病患者血清中的抗氧化状态，并探讨其与DNA氧化损伤的关系。- 本研究共纳入57例受试者。其中，32例为2型糖尿病患者，25例为非糖尿病受试者。彗星试验用于定量淋巴细胞中DNA变化的水平。采用分光光度法测定血清丙二醛（MDA）、蛋白质羰基含量、超氧化物歧化酶（SOD）、蛋白质巯基（P-SH）活性。- 彗星尾部DNA平均值显著增加，表明DNA发生变化。与对照组比较，糖尿病组与对照组比较，差异有统计学意义（P < 0. 05）。糖尿病组与对照组比较，MDA和蛋白质羰基含量显著升高，SOD和P-SH含量显著降低。血清SOD与彗星尾DNA含量呈负相关。这些结果表明糖尿病患者存在特定的脂质过氧化、蛋白质氧化和氧化DNA变化。2型糖尿病患者血液中葡萄糖稳态的紊乱与氧化剂的增加和抗氧化酶的减少有关。本研究提示，氧化-抗氧化失衡状态可能是2型糖尿病患者淋巴细胞DNA损伤的机制之一。(C)2009年由Elsevier Masson SAS出版。
Background and aim.- Diabctes mellitus is a complex Metabolic disorder characterized by a distrubance ill glucose metabolism I is Ill Recent evidence Back suggest that increased oxidative stress as well as alteration of oxidative capacity may be related to the Complications seen in patients, with type 2 diabetes. The aim Of this study, Was 10 measure serum antioxidant status in type 2 diabetic patients and to assess its relationship with oxidative DNA damage.Methods. - A total of 57 subjects were included in this study. Of these, 32 were type 2 diabetic patients and 25 were non-diabetic subjects. Comet assay was used to quantify level of DNA change in lymphocytes. Spectroscopy Methods were used to assess of serum levels Of malondialdehyde (MDA) and protein carbonyl, and serum activity of superoxide dismutase (SOD) and the protein thiol (P-SH)Results. - A significant increase in mean Comet tail DNA, indicating DNA change. was observed in diabetic patients Compared with Controls. Diatietic patients had significanfly higher levels of MDA and protein carbonyl in parallel with significant decreases in levels of SOD and the P-SH group Compared With controls. Serum SOD was also inversely Correlated with the increase in Comet tail DNA.Conclusion. -These results indicate the presence of specific lipid peroxidation, protein Oxidation and oxidative DNA changes in patients with diabetes. Perturbation Of glucose homoeostasis was associated with an increase in oxidants and,I concomitant decrease of antioxidant enzymes in the type 2 diabetic patients' blood. The present study suggests that the status of oxidant-antioxidant imbalance may be one of the mechanisms leading to the DNA damage detected in the lymphocytes of type2-diabetic patients. (C) 2009 Published by Elsevier Masson SAS.