Mining cotton fiber strength candidate genes based on transcriptome mapping
Mining cotton fiber strength candidate genes based on transcriptome mapping
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基于转录组图谱挖掘棉纤维强度候选基因
DOI：
10.1007/s11434-009-0708-z
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发表时间：
2009-12-01
影响因子：
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通讯作者：
Ma ZhiYing
中科院分区：
文献类型：
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作者：
Liu HengWei;Wang XingFen;Ma ZhiYing
棉纤维强度主要决定于次生细胞壁增厚阶段。在花后24-25天的SCW加厚期，利用AFLP技术构建纤维转录组群。在此基础上，通过数量性状基因座（QTL）扫描，利用复合区间作图法（CIM）对棉花纤维强度候选基因进行定位。作图群体为陆地棉×棉种间回交BC_1。巴巴多斯岛以115株BC 1植株为材料，用102个来自G.将78个TDFs分为8个转录组，总长度为462.63厘摩（cM）。检测到两个显著的QTL FS 1和FS 2，分别解释纤维比强度变异的16.08%和15.87%。在与FS 1和FS 2共分离的6个TDFs中，除了一个编码未知蛋白外，5个分别靶向假定的磷脂酰肌醇激酶、海藻糖-6-磷酸合酶、MADS转录因子、纤维素酶样蛋白和苯丙氨酸解氨酶。这些功能基因参与了植物细胞壁的形成或纤维素的合成代谢过程，被认为是控制棉纤维比强度的候选基因。
Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24-25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium, hirsutum x G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6-phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogen- esis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.