Cholera toxin affects nuclear ADP-ribosylation in GH1 cells.
Cholera toxin affects nuclear ADP-ribosylation in GH1 cells.
复制标题
霍乱毒素影响 GH1 细胞中的核 ADP-核糖基化。
DOI：
10.1016/0006-291x(88)90523-2
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发表时间：
1988
影响因子：
3.1
通讯作者：
Samuels,H
中科院分区：
文献类型：
--
作者：
Aranda,A;Pascual,A;Copp,R;Samuels,H
霍乱毒素对GH1细胞孵育24小时，可抑制[32P] adp核糖掺入组蛋白和非组蛋白核蛋白50%以上。在微球菌核酸酶消化后，毒素会导致所有蛋白质受体和从染色质中切除的聚（adp -核糖基）化成分的掺入量普遍减少。用霍乱毒素治疗后，NAD的细胞水平也下降了（40 - 80%）。在抑制聚（adp -核糖基）化之前，[32P] adp -核糖掺入增加，因为与毒素孵育3小时导致掺入增加而不是减少。二丁基环AMP孵生24 h也能抑制核聚（adp -核糖基）化，表明霍乱毒素的作用可能是由环AMP介导的。
Incubation of GH1 cells with cholera toxin for 24 h inhibits [32P]ADP-ribose incorporation into histones and non-histone nuclear proteins by more than 50%. The toxin produces a generalized decrease of incorporation into all protein acceptors and into the poly(ADP-ribosyl)ated components excised from chromatin after micrococcal nuclease digestion. The cellular levels of NAD were also decreased (40 to 80%) after treatment with cholera toxin. The inhibition of poly(ADP-ribosyl)ation is preceeded by an increase of [32P]ADP-ribose incorporation, since incubation with the toxin for 3 h caused an increase instead of a decrease of incorporation. Incubation with dibutyryl cyclic AMP for 24 h also inhibited nuclear poly(ADP-ribosyl)ation, thus showing that the effect of cholera toxin might be mediated by cyclic AMP.