MONOCLONAL-ANTIBODIES DEFINE 8 INDEPENDENT ANTIGENIC REGIONS ON THE BOVINE LEUKEMIA-VIRUS (BLV) ENVELOPE GLYCOPROTEIN-GP51
MONOCLONAL-ANTIBODIES DEFINE 8 INDEPENDENT ANTIGENIC REGIONS ON THE BOVINE LEUKEMIA-VIRUS (BLV) ENVELOPE GLYCOPROTEIN-GP51
复制标题
DOI：
10.1016/0042-6822(82)90234-3
复制
发表时间：
1982-01-01
期刊：
影响因子：
3.7
通讯作者：
BURNY, A
中科院分区：
文献类型：
--
作者：
BRUCK, C;MATHOT, S;BURNY, A
表征了抗BLV [牛白血病病毒] gp 51单克隆抗体（C15）。竞争性抗体结合试验表明，它们针对BLV gp 51分子上的8个独立抗原区域。这些gp 51表位中的一些的构象或可接近性随所使用的测试系统而变化，即用放射性标记抗原的液相放射免疫测定或用塑料结合的gp 51或BLV颗粒的固相酶免疫测定。使用针对2个独立表位的单克隆抗体的2位点免疫测定法允许检测最低浓度为0.4 ng/ml的分离gp 51分子，也适用于检测BLV颗粒。
Monoclonal anti-BLV [bovine leukemia virus] gp51 antibodies (C15) are characterized. Competition antibody binding assays show that they are directed against 8 independent antigenic regions on the BLV gp51 molecule. Conformation or accessibility of some of these gp51 epitopes change with the test system used, namely the liquid phase radioimmunoassay with radiolabeled antigen or the solid phase enzyme immunoassay with plastic bound gp51 or BLV particles. A 2-site immunometric assay using monoclonal antibodies directed against 2 independent epitopes allows detection of isolated gp51 molecules at a minimal concentration of 0.4 ng/ml and is also suitable for the detection of BLV particles.