Rearing environment, sex and developmental lead exposure modify gene expression in the hippocampus of behaviorally naïve animals.
Rearing environment, sex and developmental lead exposure modify gene expression in the hippocampus of behaviorally naïve animals.
复制标题
DOI：
10.1016/j.neuint.2013.01.003
复制
发表时间：
2013-03
影响因子：
4.2
通讯作者：
Schneider JS
中科院分区：
文献类型：
--
作者：
Anderson DW;Mettil WA;Schneider JS
发育性铅暴露会损害人类和动物的各种认知过程和行为。特别是，在铅暴露的大鼠中，已经描述了空间学习和记忆的特定缺陷。饲养环境（即不富集与富集）对认知表现有显著影响，饲养环境和性别可能改变铅暴露对学习记忆过程的影响。行为测试还可以改变海马体基因表达和环境的相互作用。然而，关于发育性铅暴露对海马中关键认知相关基因表达的分子相关性，以及性别和环境抚养条件如何改变这些影响，我们知之甚少。本研究检测了神经生物学相关基因（即神经营养因子、NMDA受体、代谢性谷氨酸受体、突触功能/可塑性和转录/基因调控）在围产期（即妊娠至断奶）暴露于不同水平Pb (250，750 ppm和1500ppm醋酸铅)在非强化环境（没有玩具的标准房屋）和强化环境（每周更换两次的装有玩具的大笼子）中饲养的雄性和雌性。与以往的研究不同，通过行为测试确定基因变化，从而改变表达分析，我们发现了单独暴露于铅后海马基因的性别和环境相关变化。所描述的基因表达变化可能与学习和记忆有关，并可能预先决定铅暴露后认知特征的发展。
Developmental lead (Pb) exposure impairs various cognitive processes and behaviors in both humans and animals. In particular, specific deficits in spatial learning and memory have been described in Pb-exposed rats. It is also known that rearing environment (i.e., non-enriched vs. enriched) can have significant influences on cognitive performance and that rearing environment and sex may modify the influence of Pb exposure on learning and memory processes. It is also known that behavioral testing can alter hippocampal gene expression and interactive effects of environment. Little is known however about the molecular correlates of developmental Pb-exposure on expression of key sets of cognition-relevant genes in the hippocampus and how sex and environmental rearing condition may modify these effects. The present study examined expression profiles of neurobiologically-relevant genes (i.e., neurotrophic factors, NMDA receptors, metabotropic glutamate receptors, synaptic function/plasticity, and transcription/gene regulation ) in behaviorally naïve rats with perinatal exposure (i.e., gestation through weaning) to different levels of Pb (250, 750 and 1500ppm Pb acetate) in males and females raised in a non-enriched environment (standard housing without toys) or an enriched environment (large cage containing toys changed twice weekly). Unlike previous studies identifying gene changes following behavioral testing, which alters expression analysis, we identified both sex and environmental related changes in hippocampal genes following Pb exposure alone. The gene expression changes described may be associated with learning and memory and may pre-determine how cognitive profiles develop following Pb exposure.
登录
查看更多内容
影响因子：
2.9
作者：
Guilarte, TR;McGlothan, JL
通讯作者：
McGlothan, JL
影响因子：
3.3
作者：
Corti, C;Aldegheri, L;Ferraguti, F
通讯作者：
Ferraguti, F
影响因子：
11.2
作者：
Guilarte, TR;Toscano, CD;Weaver, SA
通讯作者：
Weaver, SA
DOI：
10.1016/s0169-328x(99)00005-4
发表时间：
1999-03-20
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Nihei, MK;Guilarte, TR
通讯作者：
Guilarte, TR
DOI：
10.1093/jnen/63.6.598
发表时间：
2004-06-01
影响因子：
3.2
作者：
Keyvani, K;Sachser, N;Paulus, W
通讯作者：
Paulus, W