The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix.
The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix.
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富含半胱氨酸的肠道蛋白参与亚洲硬蛤（文蛤）的早期发育和先天免疫。
DOI：
10.1016/j.fsi.2014.07.027
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发表时间：
2014
影响因子：
4.7
通讯作者：
Song Xie
中科院分区：
文献类型：
--
作者：
Hongjian Chen;Xue Yang;T. Tang;Juan Li;Baozhong Liu;Fengsong Liu;Song Xie
富含半胱氨酸的肠蛋白（CRIP）是一种Zn2+结合蛋白，含有高度保守的双锌指结构LIM （lin-11- isl1 -mec-3）基元的一个拷贝。本文从亚洲硬蛤（meretrix meretrix）中分离到编码mmcripx的cDNA。mmcript全长cDNA由一个237 bp的开放阅读框组成，编码78个氨基酸的多肽，预测分子量（MW）为8635.8 Da，理论等电点（pI）为9.01。生物信息学分析表明，该基因属于CRIP亚家族的新成员。关系分析显示，MmCRIP与其他动物（特别是无脊椎动物）中报道的许多crip具有高度的序列相似性。Real-time PCR分析显示，mmcripp在血细胞组织和小儿期的表达水平最高。用嗜水气单胞菌攻毒成熟蛤，研究其免疫功能。在。亲水性感染后，蛤的血细胞、鳃和肝胰脏中均检测到mmcript转录上调。采用DsRNAi（双链RNA干扰）方法研究MmCRIP的功能，数据显示，themmcripp基因失活可阻断幼虫发育并导致大量死亡。本文首次提出了MmCRIP在蛤蜊早期发育和先天免疫中的可能作用。
Cysteine-rich intestinal protein (CRIP), a Zn2+-binding protein, contains a single copy of the highly conserved double-zinc-finger structure known as the LIM (lin-11-isl-1-mec-3) motif. In this paper, a cDNA encodingMmCRIPwas isolated from the Asiatic hard clamMeretrix meretrix. The full-length cDNA ofMmCRIPconsists of a 237-bp open reading frame that encodes a polypeptide of 78 amino acids with a predicted molecular weight (MW) of 8635.8 Da and theoretical isoelectric point (pI) of 9.01. Bioinformatics analysis showed that it belonged to a new member of the CRIP subfamily. Relationship analysis revealed that MmCRIP has high-levels of sequence similarity to many CRIPs reported in other animals, particularly in invertebrates. Real-time PCR analysis showed that the highest level ofMmCRIPexpression was in hemocyte tissue and at pediveligers stage. To investigate immune function, mature clams were challenged with Aeromonas hydrophila. DuringA.hydrophilainfection, up-regulation ofMmCRIPtranscript in clam's hemocyte, gill and hepatopancreas was detected. DsRNAi (double-strand RNA interference) approach was employed to study the function of MmCRIP and the data showed that inactivation of theMmCRIPgene blocked larvae development and caused mass mortalities. The probable roles of MmCRIP in clam early development and innate immunity are presented for the first time.
富含人类半胱氨酸的蛋白质。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wang,X;Lee,G;Liebhaber,SA;Cooke,NE
通讯作者：
Cooke,NE
影响因子：
6.4
作者：
Cousins, RJ;Lanningham-Foster, L
通讯作者：
Lanningham-Foster, L
DOI：
10.1073/pnas.88.21.9671
发表时间：
1991-11-01
影响因子：
11.1
作者：
HEMPE, JM;COUSINS, RJ
通讯作者：
COUSINS, RJ