Optimizing C2C12 myoblast differentiation using polycaprolactone-polypyrrole copolymer scaffolds
Optimizing C2C12 myoblast differentiation using polycaprolactone-polypyrrole copolymer scaffolds
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DOI：
10.1002/jbm.a.36556
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发表时间：
2019-01-01
影响因子：
4.9
通讯作者：
Freeman, Joseph
中科院分区：
文献类型：
--
作者：
Browe, Daniel;Freeman, Joseph
组织工程和生物材料开发的进步有可能为大体积骨骼肌缺损的问题提供可扩展的解决方案。以前关于骨骼肌再生支架开发的研究主要集中在增加电导率的策略上，这改善了卫星细胞的附着和分化。然而，这些策略通常会增加支架刚度，一些研究表明这可能对成肌细胞发育不利。在这项研究中，聚合物聚吡咯（PPy）和聚己内酯（PCL）一起合成为共聚物（PPy-PCL），旨在增加支架的导电性，而不会显着影响刚度。通过静电纺丝制备不同的支架组，表征并评估其对成肌细胞增殖和分化的适用性。这些组包括纯PCL、10% PPy-PCL、20% PPy-PCL和40% PPy-PCL的对齐和随机迭代。只有40% PPy-PCL组具有可测量的电导率，并且PPy-PCL的添加对支架的刚度没有显著影响。与纯PCL支架相比，PPy-PCL共聚物显著增加了C2 C12成肌细胞的附着，但PPy-PCL的浓度没有显著改变细胞附着。此外，与聚吡咯-PCL支架促进成肌细胞分化的程度比PCL制成的支架测量的融合指数和细胞核数每肌管。对齐的支架上级随机支架在几乎所有的措施。这些结果表明，导电性可能不是改善骨骼肌支架的关键因素。相反，细胞附着和对齐的引导信号可能对成肌细胞分化有更大的影响。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part A：107A：220-231，2019.
Advancements in tissue engineering and biomaterial development have the potential to provide a scalable solution to the problem of large-volume skeletal muscle defects. Previous research on the development of scaffolds for skeletal muscle regeneration has focused on strategies for increasing conductivity, which has improved satellite cell attachment and differentiation. However, these strategies usually increase scaffold stiffness, which some studies suggest may be detrimental to myoblast development. In this study, the polymers polypyrrole (PPy) and polycaprolactone (PCL) were synthesized together into a copolymer (PPy-PCL) designed to increase scaffold conductivity without significantly influencing stiffness. Different scaffold groups were fabricated via electrospinning, characterized, and assessed for their suitability for myoblast proliferation and differentiation. The groups included an aligned and random iteration of pure PCL, 10% PPy-PCL, 20% PPy-PCL, and 40% PPy-PCL. Only the 40% PPy-PCL group had a measureable conductivity, and the addition of PPy-PCL had no significant effect on the stiffness of the scaffolds. The PPy-PCL copolymer significantly increased the attachment of C2C12 myoblasts as compared to pure PCL scaffolds, but the concentration of PPy-PCL did not significantly alter cell attachment. In addition, scaffolds with PPy-PCL promoted myoblast differentiation to a greater extent than scaffolds made of PCL as measured by fusion index and number of nuclei per myotube. Aligned scaffolds were superior to random scaffolds in almost all measures. These results suggest that conductivity may not be the key factor in improving skeletal muscle scaffolds. Instead, cell attachment and aligned guidance cues may have a greater impact on myoblast differentiation. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 220-231, 2019.